Human IFI16-KO Dual Reporter THP-1 Cells

IFI16 knockout dual reporter monocytes

THP1-Dual™ KO-IFI16 cells were generated from THP1-Dual™ cells by stable knockout of the IFI16 (Interferon-γ-inducible protein 16) gene. IFI16 is an innate immune sensor with multiple functions; DNA sensor, regulator of interferon (IFN) expression, activator of the inflammasome, and regulator of genome function [1,2]. These cell lines were derived from human THP‑1 monocytes, a cell line often used to study DNA sensing pathways as they express all the cytosolic DNA sensors identified so far (with the exception of DAI).

THP1-Dual™ and THP1-Dual™ KO-IFI16 cells stably express two inducible secreted reporter genes: IFN-inducible Lucia luciferase and NF‑κB-inducible SEAP (secreted embryonic alkaline phosphatase). They can be used to study the role of IFI16 by monitoring IRF-induced Lucia luciferase activity, using QUANTI‑Luc™, a Lucia™ luciferase detection reagent.

THP1-Dual™ KO-IFI16 cells are resistant to the selectable markers blasticidin and Zeocin®.

References:

1. Jakobsen MR. & Paludan SR., 2014. IFI16: At the interphase between innate DNA sensing and genome regulation. Cytokine Growth Factor Rev. 25(6):649-55.
2. Unterholzner L. et al., 2010. IFI16 is an innate immune sensor for intracellular DNA. Nat Immunol. 11(11):997-1004.