THP-1 Lucia Reporter Cells (NF-κB)

Product Unit size Cat. code Docs. Qty. Price

THP1-Lucia™ NF-κB Cells

NF-κB-Lucia luciferase reporter monocytes

Show product

3-7 x 10e6 cells


NF-κB–Lucia Reporter Monocytes

Signaling pathways in THP1-Lucia™ NF-κB cells
Signaling pathways in THP1-Lucia™ NF-κB cells
(click to enlarge)

THP1-Lucia™ NF-κB cells were specifically designed for monitoring the NF-κB signal transduction pathway in a physiologically relevant cell line. THP1-Lucia™ cells were derived from the human THP-1 monocyte cell line by stable integration of an NF-κB-inducible Lucia luciferase reporter construct.

As a result, THP1-Lucia™  NF-κB cells allow the monitoring of NF-κB activation by assessing the activity of the secreted Lucia luciferase. The level of NF-κB-induced Lucia in the cell culture supernatant is readily assessed with QUANTI-Luc™ 4 Lucia/Gaussia, a luciferase detection reagent.

THP-1 cells endogenously express many pattern-recognition receptors (PRRs), including Toll-like receptors (TLRs), the stimulator of interferon genes (STING), and RIG-I-like receptors (RLR). Therefore, THP1-Lucia™ NF-κB cells are highly responsive to PRR agonists that trigger the NF-κB pathway (see figure).


Key features

  • Functionally validated with a selection of PRR ligands and cytokines
  • Readily assessable NF-κB activation by assessing the Lucia activity


NF-κB responses of THP1-Lucia™ NF-κB cells
NF-κB responses of THP1-Lucia™ NF-κB cells

NF-κB responses in THP1-Lucia™ NF-κB cells. Cells were incubated for 24 hours with various PRR ligands: Pam3CSK4 (TLR2 ligand, 10 ng/ml), Poly(I:C) HMW (TLR3 ligand, 10 µg/ml), LPS-EK Ultrapure (UP) (TLR4 ligand, 10 ng/ml), FLA-ST UP (TLR5 ligand, 10 ng/ml), Imiquimod (TLR7 ligand, 10 µg/ml), TL8-506 (TLR8 ligand, 1 µg/ml), ODN 2006 (TLR9 ligand, 10 µg/ml), Tri-DAP (NOD1 ligand, 10 µg/ml), MDP (NOD2 ligand, 10 µg/ml), 2’3’-cGAMP (STING ligand, 100 µg/ml), and 3p-hpRNA complexed with LyoVec™ (LV) (RIG-I ligand, 1 µg/ml). Human TNF-α (10 ng/ml) was used as an NF-κB-positive control. After 24h incubation, the NF-κB response was assessed by measuring the activity of Lucia luciferase in the supernatant using QUANTI-Luc™. Data are shown in fold response over non-induced cells (mean ± SEM).

Back to the top


Antibiotic resistance: Zeocin®

Growth medium: RPMI 1640, 2 mM L-glutamine, 25 mM HEPES, 10% heat-inactivated fetal bovine serum, 100 μg/ml Normocin™, Pen-Strep (100 U/ml-100 μg/ml)

Quality Control:

  • Reporter activity has been verified by functional assays.
  • The stability for 20 passages, following thawing, has been verified. 
  • These cells are guaranteed mycoplasma-free. 

These products are covered by a Limited Use License (see Terms and Conditions).

Back to the top


  • 1 vial of THP1-Lucia™ NF-kB cells (3-7 x 106 cells)
  • 1 ml of Normocin™ (50 mg/ml)
  • 1 ml of  Zeocin® (100 mg/ml)
  • 1 tube of QUANTI-Luc™ 4 Reagent, a Lucia luciferase detection reagent (sufficient to prepare 25 ml)

Dry Ice Shipped on dry ice (Europe, USA, Canada, and some areas in Asia)

Back to the top

FAQ Cell Lines

Visit our FAQ Any questions about our cell lines ? Visit our frequently asked questions page

Back to the top

Notification: A new clone is provided with an improved reporter/background signal ratio. The cat code has been changed accordingly (thpl-nfkbv2). 
This product is for internal research use only. Additional rights may be available. Please visit InvivoGen’s Terms and Conditions.

Customer Service
& Technical Support
Shopping cart is empty