THP1-Lucia™ NF-κB Cells
THP1-Lucia™ NF-κB Cells | Unit size | Cat. code | Docs | Qty | Price |
---|---|---|---|---|---|
NF-κB Luc Reporter Monocytes |
3-7 x 10e6 cells |
thpl-nfkb |
You may also need : Zeocin® | View more associated products ▼
Notification: This product is for internal research use only. Additional rights may be available. Please visit InvivoGen’s Terms and Conditions.
NF-κB Lucia™ Reporter Monocytes
THP1-Lucia™ cells were specifically designed for monitoring the NF-κB signal transduction pathway in a physiologically relevant cell line.
THP1-Lucia™ were derived from the human THP-1 monocyte cell line by stable integration of an NF-κB-inducible Luc reporter construct.
As a result, THP1-Lucia™ cells allow the monitoring of NF-κB activation by determining the activity of secreted luciferase (Lucia).
The levels of NF-κB-induced Lucia in the cell culture supernatant are readily assessed with QUANTI-Luc™ 4 Lucia/Gaussia, a luciferase detection reagent.
THP1-Lucia™ cells induce the activation of NF-κB in response to various PRR ligands.
THP1-Lucia™ cells are resistant to Zeocin®.
Back to the topSpecifications
Antibiotic resistance: Zeocin®
Growth medium: RPMI 1640, 2 mM L-glutamine, 25 mM HEPES, 10% heat-inactivated fetal bovine serum, 100 μg/ml Normocin™, Pen-Strep (100 U/ml-100 μg/ml)
Guaranteed mycoplasma-free
These products are covered by a Limited Use License (see Terms and Conditions).
Back to the topContents
- 1 vial of THP1-Lucia™ NF-kB cells (3-7 x 106 cells)
- 1 ml of Normocin™ (50 mg/ml)
- 1 ml of Zeocin® (100 mg/ml)
- 1 tube of QUANTI-Luc™ 4 Reagent, a Lucia luciferase detection reagent (sufficient to prepare 25 ml)
- 109 cells HKLM (Heat killed Listeria monocytogenes; positive control of TLR2 activity)
Shipped on dry ice (Europe, USA, Canada, and some areas in Asia)
Description
THP1-Lucia™ were derived from the human THP-1 monocyte cell line by stable integration of an NF-κB-inducible Luc reporter construct.
THP1-Lucia™ cells feature the Lucia gene, a secreted luciferase reporter gene, driven by an IFN-β minimal promoter fused to five copies of the NF-κB consensus transcriptional response element and three copies of the c-Rel binding site.
As a result, THP1-Lucia™ cells allow the monitoring of NF-κB activation by determining the activity of Lucia luciferase.