THP1-Lucia™ NF-κB Cells

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NF-κB Lucia™ Reporter Monocytes

THP1-Lucia™ cells were specifically designed for monitoring the NF-κB signal transduction pathway in a physiologically relevant cell line.

THP1-Lucia™ were derived from the human THP-1 monocyte cell line by stable integration of an NF-κB-inducible Luc reporter construct.

As a result, THP1-Lucia™ cells allow the monitoring of NF-κB activation by determining the activity of secreted luciferase (Lucia).

The levels of NF-κB-induced Lucia in the cell culture supernatant are readily assessed with QUANTI-Luc™ 4 Lucia/Gaussia, a luciferase detection reagent.

THP1-Lucia™ cells induce the activation of NF-κB in response to various PRR ligands.

THP1-Lucia™ cells are resistant to Zeocin®.

Figures

Cells were incubated with 10e7 cells/ml HKLM (TLR2), 1 ng/ml Pam3CSK4(TLR1/2), 0.1 ng/ml FSL-1 (TLR2/6), 10 µg/ml poly(I:C) (TLR3), 1 µg/ml LPS-EK UP (TLR4), 100 ng/ml FLA-ST UP (TLR5), 1 µg/ml R848 (TLR7/8), 10 µg/ml ODN2006 (TLR9), 10 µg/ml Tri-DAP (NOD1) or 10 µg/ml MDP (NOD2). After 24h incubation, the levels of NF-κB-induced Lucia luciferase were assessed from the cell culture supernatant using QUANTI-Luc™.

Specifications

Antibiotic resistance: Zeocin®

Growth medium: RPMI 1640, 2 mM L-glutamine, 25 mM HEPES, 10% heat-inactivated fetal bovine serum, 100 μg/ml Normocin™, Pen-Strep (100 U/ml-100 μg/ml)

Guaranteed mycoplasma-free

These products are covered by a Limited Use License (see Terms and Conditions).

Contents

• 1 vial of THP1-Lucia™ NF-kB cells (3-7 x 10⁶ cells)
• 1 ml of Normocin™ (50 mg/ml)
• 1 ml of  Zeocin® (100 mg/ml)
• 1 tube of QUANTI-Luc™ 4 Reagent, a Lucia luciferase detection reagent (sufficient to prepare 25 ml)
• 10⁹ cells HKLM (Heat killed Listeria monocytogenes; positive control of TLR2 activity)

Shipped on dry ice (Europe, USA, Canada, and some areas in Asia)

Description

THP1-Lucia™ were derived from the human THP-1 monocyte cell line by stable integration of an NF-κB-inducible Luc reporter construct. 
THP1-Lucia™ cells feature the Lucia gene, a secreted luciferase reporter gene, driven by an IFN-β minimal promoter fused to five copies of the NF-κB consensus transcriptional response element and three copies of the c-Rel binding site.
As a result, THP1-Lucia™ cells allow the monitoring of NF-κB activation by determining the activity of Lucia luciferase.

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