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293/Control cells

293/LacZ Unit size Cat. code Docs Qty Price
LacZ expressing 293 cell line
3-7 x 10e6 cells
293-lacz
+-
$666.00
293/null Unit size Cat. code Docs Qty Price
293 Control cell line
3-7 x 10e6 cells
293-null
+-
$666.00
293XL/null Unit size Cat. code Docs Qty Price
293XL Control cell line
3-7 x 10e6 cells
293xl-null
+-
$666.00

293/Control cells were generated by stably transfecting HEK293 cells with a pUNO control plasmid expressing either the lacZ reporter gene or a multiple cloning site.

 

• 293/LacZ cells express the lacZ reporter gene and can be used as a control cell line for 293/TLR and 293/NOD cells.

• 293/null cells contain a multiple cloning site and can be used as a control cell line for 293/TLR and 293/NOD cells.

• 293XL/null cells express the human antiapoptotic Bcl-XL gene and contain a multiple cloning site. They can be used as a control cell line for 293XL/TLR cells.

 


293/TLR, NOD and control clones were transfected transiently with pNiFty-SEAP and stimulated with TLR and NOD ligands. After 16 hour stimulation, NF-κB-induced SEAP activity was assessed using QUANTI-Blue™, a SEAP detection medium.

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Specifications

Antibiotic resistance: Blasticidin

Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™

Guaranteed mycoplasma-free

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Contents

Shipped on dry ice

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Citations

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