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293XL cell line expressing the human TLR8a gene
3-7 x 10e6 cells
HEK293 clones expressing TLR8
293XL/TLR8 cells were obtained by stable transfection of HEK293XL cells with a pUNO-TLR8 plasmid which expresses the human TLR8 gene.
The control cell line of 293XL/TLR8 cells is 293XL/null cells.
Antibiotic resistance: blasticidin
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
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TLR8 was identified together with TLR7 and TLR9 and is expressed more abundantly in PBL and lung . Human TLR8 and TLR7 were reported to independently confer responsiveness to R848, an imidazoquinolone with antiviral activity. R848 induces the activation of NF-κB in HEK293 cells transfected with human TLR8 but not in HEK293 cells expressing murine TLR8, suggesting a species specificity . Recently, single-stranded RNA (ssRNA) was identified as the natural ligand of human TLR83. GU-rich ssRNA oligonucleotides derived from HIV-1 were shown to activate DC and macrophages. TLR8 recognizes ssRNA in the endosomal compartment and leads to the secretion of cytokines .
1. Chuang TH. and RJ. Ulevitch, 2000. Cloning and characterization of a sub-family of human toll-like receptors: hTLR7, hTLR8 and hTLR9. Eur Cytokine Netw, 11(3):372-8
2. Jurk M. et al., 2002. Human TLR7 or TLR8 independently confer responsiveness to the antiviral compound R-848. Nat Immunol, 3(6):499
3. Heil F. et al., 2004. Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8. Science. 303(5663):1526-9.