CL097

TLR7/8 Agonist - Imidazoquinoline compound

CL097 is a highly water-soluble derivative of the imidazoquinoline compound R848 (Resiquimod). Like R848, CL097 induces TLR7 and/or TLR8  responses in human and murine immune cells [1, 2].

CL097 has been described as a preferential TLR7 agonist [1]. CL097 has been shown as a strong inducer of plasmacytoid dendritic cells (pDCs) activation, which represents a key therapeutic axis for cancer or other diseases [2].

We confirmed the TLR7/8 agonist activity of CL097 using our reporter cell lines HEK-Blue™ hTLR7, HEK-Blue™ hTLR8, HEK-Dual™ mTLR7, and HEK-Blue™ mTLR8. CL097 is a more potent hTLR7 agonist than other TLR7/8 agonists, including CL075, and TLR7 agonists, such as Gardiquimod, Imiquimod, Loxoribine, and CL264. However, CL097 is a less potent hTLR8 agonist than CL075 and the TLR8 agonist TL8-506. CL097 also activates murine TLR7 (mTLR7), but not mTLR8.  

Key features of CL097:

• Agonist of hTLR7 and hTLR8 with higher potency towards hTLR7.
• Agonist of mTLR7.
• Each lot of CL097 is highly pure (>95%) and functionally tested.

References:

1. Schön M.P. & Schön M., 2008. TLR7 and TLR8 as targets in cancer therapy. Oncogene. 27:190-199.
2. Wu J. et al., 2019. pDC activation by TLR7/8 ligand CL097 compared to TLR7 ligand IMQ or TLR9 ligand CpG.  J. Immunol. Res. ID:1749803.

Specifications

Specificity: human TLR7/8 and mouse TLR7 agonist

CAS number: 1026249-18-2

Formula: C₁₃H₁₄N₄O 

Molecular weight: 242.28 g/mol

Solubility: 1 mg/ml in water

Working Concentration:

• 0.3 - 3 μg/ml CL097 for human TLR8 and mouse TLR7 in cell culture assays
• 50 ng - 3 μg/ml CL097 for human TLR7 in cell culture assays

Quality control:

• Purity: ≥95% (UHPLC)
• The biological activity of CL097 has been verified using cellular assays.
• The absence of bacterial contamination has been confirmed using HEK-Blue™ hTLR2 cells (for lipoproteins) and HEK-Blue™ hTLR4 cells (for endotoxins).

Contents

CL097 is available in two quantities:

tlrl-c97:

• 500 µg CL097
• 1.5 ml of sterile endotoxin-free water

tlrl-c97-5:

• 5 mg CL097
• 10 ml of sterile endotoxin-free water

CL097 is provided as a pale yellow solid and shipped at room temperature.

Store at -20 ̊C.

Lyophilized product is stable for 1 year at -20°C when properly stored.

Resuspended CL097 should be aliquoted and stored at -20 ̊C.

Resuspended product is stable 6 months at -20°C.

 Avoid repeated freeze-thaw cycles.

Details

Chemical structure of CL097: