HEK-Blue™ hTLR8
HEK-Blue™ hTLR8 cells | Unit size | Cat. code | Docs | Qty | Price |
---|---|---|---|---|---|
Human TLR8-expressing HEK 293 cells |
3-7 x 10e6 cells |
hkb-htlr8 |
You may also need : HEK-Blue™ Null1 Cells | View more associated products ▼
Human TLR8 / NF-κB / SEAP reporter HEK293 cells
TLR8 activation in HEK-Blue™ hTLR8 cells
HEK-Blue™ hTLR8 cells were derived from the human embryonic kidney HEK293 cell line to monitor the activation of human TLR8. The
Structural analyses have revealed that TLR8 possesses two binding sites with distinct specificities.
- Site 1 binds uridine or synthetic base analogs, such as R848 (Resiquimod).
- Site 2 binds ssRNA with uridine (U) and guanosine (G) motifs. Both uridine and U(G) ssRNA appear to arise from RNA-degradation [5].
Of note, Site 1 occupancy allows the receptor dimerization and signaling with ad hoc ligand concentration. ssRNA-binding to Site 2 is not sufficient for the formation of a signaling competent TLR dimer but it strongly enhances the binding affinity of Site 1 [5].
HEK-Blue™ hTLR8 cells express the human TLR8 gene and an NF-κB/AP-1-inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene. SEAP levels produced upon TLR8 stimulation can be readily determined by performing the assay in HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP. Alternatively, SEAP activity may be monitored using QUANTI-Blue™, a SEAP detection reagent.
The parental cell line of HEK-Blue™ hTLR8 cells is HEK-Blue™ Null1 cells.
HEK-Blue™ hTLR8 cells are selectable with Blasticidin and Zeocin®.
References:
1. Georg P. & Sander L.E., 2019. Innate sensors that regulate vaccine responses. Curr. Op. Immunol. 59:31.
2. Heil F. et al., 2004. Species-specific recognition of single-stranded RNA via Toll-like receptor 7 and 8. Science. 303:1526.
3. Eigenbrod T. & Dalpke A.H., 2015. Bacterial RNA: an underestimated stimulus for innate immune responses. J. Immunol. 195:411
4. Choo M.K. et al., 2017. TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects. J. Exp. Med. 214:1297.
5. Tanji H. et al., 2015. Toll-like receptor 8 senses degradation products of single-stranded RNA. Nat. Struct. Mol. Biol. 22:109.
Specifications
Antibiotic resistance: Blasticidin, Zeocin®.
Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™.
Quality Control:
- The expression of the human TLR8 gene has been confirmed by RT-PCR.
- The activation of NF-κB/AP1 upon TLR8 stimulation has been verified using functional assays.
- The stability for 20 passages, following thawing, has been verified.
- These cells are guaranteed mycoplasma-free.
Note: HEK293 cells express endogenous levels of TLR3, TLR5, and NOD1. The appropriate parental cell line for HEK-Blue™ hTLR7 cells is HEK-Blue™ Null1 cells.
All of these products are covered by a Limited Use License (See Terms and Conditions).
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- 1 vial containing 3-7 x 106 cells
- 1 ml Blasticidin (10 mg/ml)
- 1 ml Zeocin® (100 mg/ml)
- 1 ml Normocin™ (50 mg/ml)
- 1 pouch of HEK-Blue™ Detection (cell culture medium for real-time detection of SEAP)
Shipped on dry ice (Europe, USA, Canada and some areas in Asia)