HEK-Blue™ hTLR8
| HEK-Blue™ hTLR8 cells | Unit size | Cat. code | Docs | Qty | Price |
|---|---|---|---|---|---|
human TLR8-expressing HEK 293 cells |
3-7 x 10e6 cells |
hkb-htlr8 |
HEK-Blue™-hTLR8 cells were obtained by co-transfection of the human TLR8 gene and an inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene into HEK293 cells.
The SEAP gene is placed under the control of the IFN-β minimal promoter fused to five NF-κB and AP-1-binding sites. Stimulation with a TLR8 ligand activates NF-κB and AP-1 which induce the production of SEAP.
Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP.
The parental cell line of HEK-Blue™ hTLR8 cells is HEK-Blue™ Null1 cells.
Specifications
Antibiotic resistance: blasticidin, Zeocin™.
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™.
Guaranteed mycoplasma-free.
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- 1 vial containing 3-7 x 106 cells
- 100 μl Blasticidin (10 mg/ml)
- 100 μl Zeocin™ (100 mg/ml)
- 1 ml Normocin™ (50 mg/ml)
- 1 pouch of HEK-Blue™ Detection (cell culture medium for real-time detection of SEAP)
Shipped on dry ice