|ssRNA40 / LyoVec™||Unit size||Cat. code||Docs||Qty||Price|
4 x 25 µg
TLR8 Agonist - HIV-1 LTR-derived ssRNA / LyoVec™
ssRNA40 is a 20-mer phosphorothioate protected single-stranded RNA oligonucleotide containing a GU-rich sequence. ssRNA40, also known as R-1075, is a U-rich single-stranded RNA derived from the HIV-1 long terminal repeat. ssRNA40 is complexed with the cationic lipid LyoVec™, to protect it from degradation and facilitate its uptake.
Specificity: human TLR8/mouse TLR7 agonist
Working Concentration: 0.25-5 μg/ml
Sequence: ssRNA40 5’-GsCsCsCsGsUsCsUsGsUsUsGsUsGsUsGsAsCsUsC-3’
(“s” depicts a phosphothioate linkage)
Endotoxin level: EndoFit™ (<0.001 EU/µg)
Solubility: 0.05 mg/ml in waterBack to the top
• 4x 25 μg lyophilized ssRNA40/LyoVec™ 1:2 ratio (w/w)
Note: Each vial contains 25 μg of ssRNA40 complexed with 50 μg LyoVec™.
• 10 ml endotoxin-free water
ssRNA40/LyoVec™ is provided lyophilized and shipped at room temperature. Store at -20 ̊C. Lyophilized product is stable 1 year at -20 ̊C. Upon resuspension, store product at 4°C. Resuspended product is stable 1 week at 4°C.Back to the top
ssRNA40 is a 20-mer phosphothioate protected single-stranded RNA oligonucleotide containing a GU-rich sequence . ssRNA40 is complexed with the cationic lipid LyoVec™, to protect it from degradation and facilitate its uptake, and lyophilized to generate ssRNA40/LyoVec™. When complexed to cationic lipids, ssRNA can substitute for viral RNAs in inducing TNF-α and IFN-α production in peripheral blood mononuclear cells [1, 2]. Murine dendritic cells deficient for TLR7 failed to produce IFN-α in response to ssRNA40, while the response to CpG-ODNs was unaffected, suggesting that TLR7 plays a critical role in viral ssRNA recognition .
In human cells, TLR8 was shown to be the key receptor for viral ssRNA, implying a species specificity difference in ssRNA recognition. During infection, some viral particles are degraded by the endosomal proteases, exposing the viral genome and allowing TLR7 and/or TLR8 signaling, which are known to occur in endosomes . TLR7 and TLR8 can recognize both self and viral RNA but seem able to distinguish the presence of viral RNA by detecting their abnormal localization in the endosome rather than a particular RNA motif.
1. Heil F. et al., 2004. Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8. Science. 5;303(5663):1526-9.
2. Diebold SS. et al., 2004. Innate antiviral responses by means of TLR7-mediated recognition of single-stranded RNA. Science. 5;303(5663):1529-31
3. Heil F. et al., 2003 The Toll-like receptor 7 (TLR7)-specific stimulus loxoribine uncovers a strong relationship within the TLR7, 8 and 9 subfamily. Eur J Immunol. 33(11):2987-97.