ssRNA40 / LyoVec™
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Cat.code:
tlrl-lrna40
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ABOUT
TLR7/8 Agonist - HIV-1-derived ssRNA - complexed with LyoVec™
ssRNA40 is a 20-mer phosphorothioate-protected single-stranded RNA oligonucleotide containing a GU-rich sequence. ssRNA40, also known as R-1075, is a U-rich single-stranded RNA derived from the long terminal repeat of HIV-1.
ssRNA derived from HIV-1 or the influenza virus were shown to induce the production of proinflammatory cytokines by activation of the Toll-like receptors TLR7 and/or TLR8.
Description
InvivoGen's ssRNA40/LyoVec™ is complexed with the cationic lipid LyoVec™ to protect it from degradation and facilitate its uptake. Moreover, phosphorothioate linkages were incorporated in order to extend the effective molecular lifetime by minimizing extra and intracellular nuclease degradation. ssRNA40/LyoVec™ is a weak TLR7 and strong TLR8 agonist, when tested using our HEK-Blue™ reporter cell lines expressing human or mouse TLR7 or TLR8 (see figure).
Key features of ssRNA40/LyoVec™
- Activator of TLR7 and TLR8
- Negative control ssRNA41/LyoVec™ is available
- Complexed with LyoVec™
- Each lot of ssRNA40/LyoVec™ is functionally tested
All products are for research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
TLR8
Human
5’-G*C*C*C*G*U*C*U*G*U*U*G*U*G*U*G*A*C*U*C-3’
* depicts a phosphothioate linkage
0.25 - 5 μg/ml
0.05 mg/ml in water
Negative (tested using EndotoxDetect™ assay)
Cellular assays
TLR8 activation
Each lot is functionally tested and validated.
Complexed with transfection reagent LyoVec™
CONTENTS
Contents
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Product:ssRNA40 / LyoVec™
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Cat code:tlrl-lrna40
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Quantity:4 x 25 µg
10 ml endotoxin-free water
Each vial contains 25 μg of ssRNA40 complexed with 50 μg LyoVec™.
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
Details
ssRNA40 is a 20-mer phosphothioate-protected single-stranded RNA oligonucleotide containing a GU-rich sequence [1].
ssRNA40 is complexed with the cationic lipid LyoVec™, to protect it from degradation and facilitate its uptake, and lyophilized to generate ssRNA40/LyoVec™. When complexed to cationic lipids, ssRNA can substitute for viral RNAs in inducing TNF-α and IFN-α production in peripheral blood mononuclear cells [1, 2].
Murine dendritic cells deficient for TLR7 failed to produce IFN-α in response to ssRNA40. In contrast, the response to CpG-ODNs was unaffected, suggesting that TLR7 plays a critical role in viral ssRNA recognition [1].
In human cells, TLR8 was shown to be the key receptor for viral ssRNA, implying a species specificity difference in ssRNA recognition.
During infection, some viral particles are degraded by the endosomal proteases, exposing the viral genome and allowing TLR7 and/or TLR8 signaling, known to occur in endosomes [3].
TLR7 and TLR8 can recognize both self and viral RNA but can distinguish the presence of viral RNA by detecting their abnormal localization in the endosome rather than a particular RNA motif.
1. Heil F. et al., 2004. Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8. Science. 5;303(5663):1526-9.
2. Diebold SS. et al., 2004. Innate antiviral responses by means of TLR7-mediated recognition of single-stranded RNA. Science. 5;303(5663):1529-31
3. Heil F. et al., 2003 The Toll-like receptor 7 (TLR7)-specific stimulus loxoribine uncovers a strong relationship within the TLR7, 8 and 9 subfamily. Eur J Immunol. 33(11):2987-97.
DOCUMENTS
Documents
Technical Data Sheet
Validation Data Sheet
Safety Data Sheet
Certificate of analysis
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