TLR-expressing NF-κB–SEAP reporter cells
HEK-Blue™ TLR cells is a collection of HEK 293 engineered cell lines designed to provide a rapid, sensitive, and reliable method to screen and validate TLR agonists or antagonists.
HEK-Blue™ TLR cells stably co-express a human or murine TLR gene and an NF-κB-inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene. SEAP levels produced upon TLR stimulation can be readily determined by performing the assay in HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP. Alternatively, SEAP activity may be monitored using QUANTI-Blue™, a SEAP detection reagent.
These cell lines are extensively tested for viability, stability, biological activity, and absence of mycoplasma to ensure strong and reproducible results. Moreover, we provide detailed handling and experimental procedures for all cell lines, to minimize the end-user's need for optimization or troubleshooting.
Key features
- Stable expression of a human or mouse TLR
- Stable expression of an NF-κB/AP1-inducible SEAP reporter gene
- Strong response to specific PRR-ligands
- Each lot is functionally tested and guaranteed mycoplasma-free
Applications
- Defining the role of TLR-dependent IRF and NF-κB signaling pathways
- Screening for novel TLR agonists and inhibitors
- Comparing the TLR-dependent signaling between human and mouse TLR
