HEK-Blue™ TLR8 cells are designed for studying the stimulation of TLR8 by monitoring the activation of NF-κB and AP-1.
TLR8 was identified together with TLR7 and TLR9 . Human TLR8 and TLR7 were reported to independently confer responsiveness to R848, an imidazoquinolone with antiviral activity.
R848 induces the activation of NF-κB in HEK293 cells transfected with human TLR8 but not in HEK293 cells expressing murine TLR8, suggesting a species specificity .
Sequence-specific single-stranded RNA (ssRNA) was identified as the natural ligand of human TLR8 . GU-rich ssRNA oligonucleotides derived from HIV-1 were shown to activate dendritic cells and macrophages.
TLR8 recognizes ssRNA in the endosomal compartment and leads to the secretion of cytokines .
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