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HEK-Blue™ TLR8

HEK-Blue™ TLR8 cells are designed for studying the stimulation of TLR8 by monitoring the activation of NF-κB and AP-1.

TLR8 was identified together with TLR7 and TLR9 [1]. Human TLR8 and TLR7 were reported to independently confer responsiveness to R848, an imidazoquinolone with antiviral activity. R848 induces the activation of NF-κB in HEK293 cells
transfected with human TLR8 but not in HEK293 cells expressing murine TLR8, suggesting a species specificity [2]. Sequence-specific single-stranded RNA (ssRNA) was identified as the natural ligand of human TLR8 [3]. GU-rich ssRNA oligonucleotides derived from HIV-1 were shown to activate dendritic cells and macrophages. TLR8 recognizes ssRNA in the endosomal compartment and leads to the secretion of cytokines [3].

1. Chuang TH. and RJ. Ulevitch, 2000. Cloning and characterization of a sub-family of human toll-like receptors: hTLR7, hTLR8 and hTLR9. Eur Cytokine Netw, 11(3):372-8.
2. Jurk M. et al., 2002. Human TLR7 or TLR8 independently confer responsiveness to the antiviral compound R-848. Nat Immunol, 3(6):499.
3. Heil F. et al., 2004. Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8. Science. 303(5663):1526-9.

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