|HEK-Blue™ mTLR8 cells||Unit size||Cat. code||Docs||Qty||Price|
murine TLR8-expressing HEK 293 cells
3-7 x 10e6 cells
SEAP Reporter 293 cells expressing the mouse TLR8 gene
HEK-Blue™-mTLR8 cells were obtained by co-transfection of the murine TLR8 gene and an inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene into HEK293 cells.
The SEAP gene is placed under the control of the IFN-β minimal promoter fused to five NF-κB and AP-1-binding sites. Stimulation with a TLR8 ligand activates NF-κB and AP-1 which induce the production of SEAP.
Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP.
The parental cell line of HEK-Blue™ mTLR8 cells is HEK-Blue™ Null1-v cells.Back to the top
Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™.
Guaranteed mycoplasma-free.Back to the top