Invivogen
Menu

Human TLR3 Reporter HEK293 Cells (NF-κB)

Product Unit size Cat. code Docs. Qty. Price

HEK-Blue™ hTLR3 cells

Human TLR3 expressing HEK293 reporter cells (NF-κB pathway)

Show product

3-7 x 10e6 cells

hkb-htlr3
+-
$1,457

Notification:  This product is for internal research use only. Additional rights may be available. Please visit InvivoGen’s Terms and Conditions.

NF-κB–SEAP reporter HEK293 cells expressing human TLR3

Signaling pathways in HEK-Blue™ hTLR3 cells
Signaling pathways in HEK-Blue™ hTLR3 cells

HEK-Blue™ hTLR3 cells were engineered from the human embryonic kidney HEK293 cell line to study the human Toll-like receptor 3 (hTLR3). This important pattern recognition receptor (PRR) recognizes double-stranded (ds)RNA, a hallmark of viral replication, and triggers antiviral immune responses [1].

More details More details

 

Description

HEK-Blue™ hTLR3 cells feature the stable expression of the human TLR3 gene as well as an inducible reporter gene for SEAP (secreted embryonic alkaline phosphatase). SEAP levels produced upon TLR3 stimulation can be readily determined by performing the assay in HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP. Alternatively, SEAP activity may be monitored using QUANTI-Blue™, a SEAP detection reagent.

HEK-Blue™ hTLR3 cells are highly responsive to synthetic analogs of dsRNA. They show potent NF-κB response upon incubation with TLR3-specific ligands, such as Poly(I:C) (polyinosinic-polycytidylic acid) or Poly(A:U) (polyadenylic–polyuridylic acid), when compared to their parental cell line HEK-Blue™ Null1 (see figures)

Of note, HEK293 cells express endogenous levels of various PRRs, including TLR3, TLR5, and RIG-I-like receptors, and therefore might respond to their cognate ligands (see figures)

 

Key features:

  • Stable expression of human TLR3
  • Strong response to dsRNA and analogs
  • Distinct monitoring of TLR3-dependent NF-κB activation by assessing the SEAP activities

Applications:

  • Defining the role of TLR3-dependent NF-κB signaling pathway
  • Screening for novel TLR3 agonists and inhibitors in comparison with their parental cell line HEK-Blue™ Null1 


References

1. Chen Y, et al., 2021.  Toll-like receptor 3 (TLR3) regulation mechanisms and roles in antiviral innate immune responses. J Zhejiang Univ Sci B.;22(8):609-632. 

Figures

Response of HEK-Blue™-derived cells to TLR3 agonists
Response of HEK-Blue™-derived cells to TLR3 agonists

Response of HEK-Blue™-derived cells to TLR3 agonists. HEK-Blue™ Null1 and HEK-Blue™ hTLR3 cells were cultured in HEK-Blue™ Detection reagent and stimulated for 24 hours with 1 µg/ml of the following TLR3 agonists: Poly(I:C) HMW, Poly(I:C) LMW, and Poly(A:U). After 24h incubation, the NF-κB-induced SEAP activity was assessed by measuring the SEAP level in the supernatant. Data are shown as optical density (OD) at 650 nm (mean ± SEM).

Dose-response of HEK-Blue™ hTLR3 cells to TLR3 agonists
Dose-response of HEK-Blue™ hTLR3 cells to TLR3 agonists

D​ose-response of HEK-Blue™ hTLR3 cells to TLR3 agonists. HEK-Blue™ hTLR3 cells were cultured in HEK-Blue™ Detection reagent and stimulated with increasing concentrations of Poly(I:C) HMW, Poly(I:C) LMW, and Poly(A:U). After 24h incubation, the NF‑κB‑induced SEAP activity was determined by reading the OD at 650 nm (mean ± SEM).

Response of HEK-Blue™hTLR3 cells to various PRR agonists and cytokines
Response of HEK-Blue™hTLR3 cells to various PRR agonists and cytokines

Response of HEK-Blue™hTLR3 cells to various PRR agonists and cytokines. Cells were cultured in HEK-Blue™ Detection reagent and stimulated for 24 hours with cytokines and various TLR agonists: Human TNF-α (NF-κB-positive control, 1 ng/ml), Pam3CSK4 (TLR2 ligand, 100 ng/ml), Poly(I:C) HMW (TLR3 ligand, 100 ng/ml), LPS-EK Ultrapure (UP) (TLR4 ligand, 100 ng/ml), FLA-ST UP (TLR5 ligand, 100 ng/ml), R848 (TLR7/8 ligand, 10 µg/ml), ODN 2006 (TLR9 ligand, 10 µg/ml), Tri-DAP (NOD1 ligand, 100 ng/ml), and MDP (NOD2 ligand, 100 ng/ml). After 24h incubation, the NF-κB-induced SEAP activity was assessed by measuring the SEAP level in the supernatant. Data are shown as OD at 650 nm (mean ± SEM).

Back to the top

Specifications

Antibiotic resistance: Blasticidin, Zeocin®

Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™

Quality Control:

  • Stable expression of human (h)TLR3 has been verified by RT-qPCR and functional assays.
  • The activation of NF-κB/AP1 upon TLR3 stimulation has been verified using functional assays.
  • The stability for 20 passages, following thawing, has been verified. 
  • These cells are guaranteed mycoplasma-free.

Note: HEK293 cells express endogenous levels of TLR3, TLR5, and NOD1.
The appropriate parental cell line for HEK-Blue™ hTLR3 cells is HEK-Blue™ Null1

 

All of these products are covered by a Limited Use License (See Terms and Conditions).

Back to the top

Contents

Dry ice shipping Shipped on dry ice (Europe, USA, Canada, and some areas in Asia)

Back to the top

Details

Toll-Like Receptor 3

In humans, four Toll-Like Receptor (TLR) family members TLR3, TLR7, TLR8, and TLR9 are specialized in sensing viral-derived components and are mainly found in the endosome. Among these, TLR3 recognizes double-stranded (ds)RNA, a hallmark of viral replication, and triggers antiviral immune responses [1]. TLR3 is expressed in myeloid dendritic cells, macrophages, as well as non-immune cells [2].

TLR3 signaling

TLR3 activation upon viral infection involves several steps, including translocation of TLR3 from the ER (endoplasmic reticulum) via the Golgi to the endosome, proteolytic cleavage and dimerization of TLR3, and finally receptor-ligand binding [3]. In order to start the signaling cascade, activated TLR3 recruits the adaptor protein TRIF (TIR domain-containing adapter-inducing interferon-β). TRIF binds to TRAF3 (TNF receptor-associated factor 3), which then recruits TBK1 (TANK-binding kinase 1) and IKKε (IκB kinase ε), thus activating the transcription factor IRF3 (interferon regulatory factor 3) and stimulating the production of type I IFNs (interferons). Additionally, TRIF interacts with TRAF6 and RIP1 (kinase receptor-interacting protein 1). RIP1 in turn binds to TAK1 (transforming growth factor β-activated kinase 1) and IKK. TAK1 phosphorylates IKKα and IKKβ, leading to the phosphorylation of IκB, the NF-κB inhibitor. Ultimately, this leads to the release and translocation of NF-κB into the nucleus and the induction of pro-inflammatory cytokines [2,4]. 

Pathology

Given its important role in dsRNA recognition, TLR3 signaling has been intensively studied. Various TLR3-agonists, such as the synthetic dsRNA analog Poly(I:C) are being used in vaccine development and cancer therapy [4]. Yet, recent studies have indicated that TLR3 may act as a double-edged sword by showing both protective and damaging functions in the context of some human viral infections [3,5]. Moreover, rare mutations in TLR3 have been associated with viral susceptibility; specifically, infections with HSV-1 (herpes simplex virus 1), influenza, and SARS-Co-V2 have been linked to pathogenic germline variants in TLR3 pathway genes [2]. Understanding the TRIF-dependent TLR3 pathway may be essential for the establishment of specific therapeutic approaches to diminish TLR3-driven disease and exploit its protective functions [3].

 

 

References

1. Manuela Sironi, et al., 2012. A Common Polymorphism in TLR3 Confers Natural Resistance to HIV-1 Infection. J Immunol 15; 188 (2): 818–823. 
2. Aluri, J, et al., 2021. Toll-Like Receptor Signaling in the Establishment and Function of the Immune System. Cells, 10, 1374.
3. Chen Y, et al., 2021.  Toll-like receptor 3 (TLR3) regulation mechanisms and roles in antiviral innate immune responses. J Zhejiang Univ Sci B.;22(8):609-632.
4. Komal A, et al., 2021. TLR3 agonists: RGC100, ARNAX, and poly-IC: a comparative review. Immunol Res. 69(4):312-322. 
5. Perales-Linares R, Navas-Martin S. 2013. Toll-like receptor 3 in viral pathogenesis: friend or foe? Immunology.;140(2):153-67.

Back to the top

FAQ Cell Lines

Visit our FAQ Any questions about our cell lines ? Visit our frequently asked questions page

Back to the top
Customer Service
& Technical Support
Shopping cart is empty