HEK-Dual™ hTLR3
HEK-Dual™ hTLR3 (NF/IL8) | Unit size | Cat. code | Docs | Qty | Price |
---|---|---|---|---|---|
Double readout TLR cells |
3-7 x 10e6 cells |
hkd-htlr3ni |
You may also need : HEK-Dual™ Null | View more associated products ▼
Human TLR3 (NF-κB-SEAP / KI-[IL-8]Lucia) dual-reporter HEK293 cells
HEK-Dual™ hTLR3 (NF/IL8) cells were generated from HEK-Dual™ Null cells by stable transfection of the human TLR3 (hTLR3) gene. Due to the knockout of TLR5, these cells enable the study of hTLR3 signaling without interference from other TLRs.
They respond to very low concentrations of TLR3 agonists such as poly(I:C) a synthetic analog of double-stranded RNA. They do not respond to other TLR agonists or to the cytokine TNF-α (see validation sheet).
HEK-Dual™ hTLR3 (NF/IL8) cells are resistant to hygromycin B, Zeocin® and blasticidin. They should be maintained in growth medium supplemented with hygromycin B and Zeocin®.
References:
1. Ohta K. et al., 2014. TLR-mediated interleukin-8 production by human submandibular gland epithelial cells. Mol Med Rep. 10(5):2377-82.
2. Roebuck KA. et al., 1999. Regulation of interleukin-8 gene expression. J Interferon Cytokine Res. 19(5):429-38.
Specifications
Antibiotic resistance: blasticidin, hygromycin, Zeocin®
Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
These cells are covered by a Limited Use License (See Terms and Conditions).
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- 1 vial containing 3-7 x 106 cells
- 1 ml of Hygromycin B Gold (100 mg/ml)
- 1 ml of Zeocin® (100mg/ml)
- 1 ml of Normocin™ (50 mg/ml)
- 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution, a SEAP detection reagent)
- 1 tube of QUANTI-Luc™ 4 Reagent, a Lucia luciferase detection reagent (sufficient to prepare 25 ml)
Shipped on dry ice (Europe, USA, Canada, and some areas in Asia)
Details
EC50 values calculated for the NF-kB response using QUANTI-Blue™. HEK-Dual™ hTLR3 (NF/IL8) and HEK-Blue™ hTLR3 cells were stimulated for 24 hours with various TLR3 agonists: Poly(I:C) HMW, Poly(I:C) LMW and Poly(A:U).
Cell line | EC50 for Poly(I:C) HMW (ng/ml) | EC50 for Poly(I:C) HMW (ng/ml) | EC50 for Poly(A:U) (ng/ml) |
HEK-Dual™ hTLR3 (NF/IL8) | 28.9 ± 17.7 | 59.4 ± 35.3 | 53.6 ± 22.9 |
HEK‑Blue™ hTLR3 | 77.6 ± 29.0 | 163.8 ± 99.9 | 218.5 ± 82.7 |