HEK-Dual™ Cells
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Cat.code:
hkd-nfis
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ABOUT
NF-κB–SEAP and IRF–Lucia reporter HEK293 cells
HEK-Dual™ cells are derived from the human embryonic kidney 293 (HEK293) cell line through the stable integration of two inducible reporter genes for SEAP (secreted embryonic alkaline phosphatase) and Lucia luciferase.
As a result, these cells allow the simultaneous study of the NF-κB pathway, by monitoring the activity of SEAP, and the IRF pathway, by assessing the activity of the secreted Lucia luciferase. Both reporter proteins are readily measurable in the cell culture supernatant when using QUANTI-Blue™ Solution, a SEAP detection reagent, and QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia luciferase detection reagent.
HEK-Dual™ cells express endogenous levels of various pattern recognition receptors (PRRs), including TLR3, TLR5, NOD1, RIG-I-like receptors (RLR), and STING [in-house data]. They induce the activation of NF-κB in response to cognate TLR/NOD ligands including Poly(I:C), flagellin, and Tri-DAP. A IRF response is triggered upon stimulation with RLR- and STING-ligands such as 3p-hpRNA and 2’3’-cGAMP, respectively (see figures).
HEK-Dual™ is the control cell line of:
- HEK-Dual™ hTLR3 cells
- HEK-Dual™ hTLR4 cells
- HEK-Dual™ hTLR7 cells
- HEK-Dual™ hTLR8 cells
- HEK-Dual™ hTLR9 cells
Key features
- Functionally validated with a selection of PRR ligands and cytokines
- Readily assessable NF-κB or IRF activation by assessing the SEAP and Lucia luciferase activities
Disclaimer: These cells are for internal research use only and are covered by a Limited Use License (See Terms and Conditions). Additional rights may be available.
SPECIFICATIONS
Specifications
Screening of PRR agonists or inhibitors
Complete DMEM (see TDS)
Verified using Plasmotest™
Each lot is functionally tested and validated.
CONTENTS
Contents
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Product:HEK-Dual™ Cells
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Cat code:hkd-nfis
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Quantity:3-7 x 10^6 cells
- 1 ml Blasticidin (10 mg/ml)
- 1 ml Normocin™ (50 mg/ml)
- 1 ml Zeocin® (100 mg/ml)
- 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution)
- 1 tube of QUANTI-Luc™ 4 Reagent (sufficient to prepare 25 ml)
Shipping & Storage
- Shipping method: Dry ice
- Liquid nitrogen vapor
- Upon receipt, store immediately in liquid nitrogen vapor. Do not store cell vials at -80°C.
Storage:
Caution:
DOCUMENTS
Documents
Technical Data Sheet
Validation Data Sheet
Safety Data Sheet
Certificate of analysis
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