NF-κB and ISG Dual Reporter HEK 293 Cells - HEK-Dual™ Cells

NF-κB-SEAP and IRF-Lucia Reporter Cells

ABOUT

NF-κB–SEAP and IRF–Lucia reporter HEK293 cells

HEK-Dual™ cells are derived from the human embryonic kidney 293 (HEK293) cell line through the stable integration of two inducible reporter genes for SEAP (secreted embryonic alkaline phosphatase) and Lucia luciferase.

As a result, these cells allow the simultaneous study of the NF-κB pathway, by monitoring the activity of SEAP, and the IRF pathway, by assessing the activity of the secreted Lucia luciferase. Both reporter proteins are readily measurable in the cell culture supernatant when using QUANTI-Blue™ Solution, a SEAP detection reagent, and QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia luciferase detection reagent. 

 

HEK-Dual™ cells express endogenous levels of various pattern recognition receptors (PRRs), including TLR3, TLR5, NOD1, RIG-I-like receptors (RLR), and STING [in-house data]. They induce the activation of NF-κB in response to cognate TLR/NOD ligands including Poly(I:C), flagellin, and Tri-DAP. A IRF response is triggered upon stimulation with RLR- and STING-ligands such as 3p-hpRNA and 2’3’-cGAMP, respectively (see figures).

HEK-Dual™ is the control cell line of:

 

Key features

  • Functionally validated with a selection of PRR ligands and cytokines
  • Readily assessable NF-κB or IRF activation by assessing the SEAP and Lucia luciferase activities

Disclaimer:  These cells are for internal research use only and are covered by a Limited Use License (See Terms and Conditions). Additional rights may be available.

SPECIFICATIONS

Specifications

Species
Human
Tested applications

Screening of PRR agonists or inhibitors

Cell type
Epithelial
Growth properties
Adherent
Tissue origin
Human embryonic kidney cells
Reporter gene
SEAP
Lucia®
Detection method
Colorimetric (SEAP), Bioluminescence (Lucia)
Antibiotic resistance
Blasticidin
Zeocin®
Growth medium

Complete DMEM (see TDS)

Mycoplasma-free

Verified using Plasmotest™

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    HEK-Dual™ Cells
  • Cat code: 
    hkd-nfis
  • Quantity: 
    3-7 x 10^6 cells
Includes:
  • 1 ml Blasticidin (10 mg/ml)
  • 1 ml Normocin™ (50 mg/ml)
  • 1 ml Zeocin® (100 mg/ml)
  • 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution)
  • 1 tube of QUANTI-Luc™ 4 Reagent (sufficient to prepare 25 ml)

Shipping & Storage

  • Shipping method:  Dry ice
  • Storage:

    • Liquid nitrogen vapor
    Stability: 20 passages

    Caution:

    • Upon receipt, store immediately in liquid nitrogen vapor. Do not store cell vials at -80°C.

DOCUMENTS

Documents

HEK-Dual™ Cells

Technical Data Sheet

Validation Data Sheet

Safety Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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