NF-κB and ISG Dual Reporter HEK 293 Cells - HEK-Dual™ Cells

NF-κB–SEAP and IRF–Lucia reporter HEK293 cells

HEK-Dual™ cells are derived from the human embryonic kidney 293 (HEK293) cell line through the stable integration of two inducible reporter genes for SEAP (secreted embryonic alkaline phosphatase) and Lucia luciferase.

As a result, these cells allow the simultaneous study of the NF-κB pathway, by monitoring the activity of SEAP, and the IRF pathway, by assessing the activity of the secreted Lucia luciferase. Both reporter proteins are readily measurable in the cell culture supernatant when using QUANTI-Blue™ Solution, a SEAP detection reagent, and QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia luciferase detection reagent. 

HEK-Dual™ cells express endogenous levels of various pattern recognition receptors (PRRs), including TLR3, TLR5, NOD1, RIG-I-like receptors (RLR), and STING [in-house data]. They induce the activation of NF-κB in response to cognate TLR/NOD ligands including Poly(I:C), flagellin, and Tri-DAP. A IRF response is triggered upon stimulation with RLR- and STING-ligands such as 3p-hpRNA and 2’3’-cGAMP, respectively (see figures).

HEK-Dual™ is the control cell line of:

• HEK-Dual™ hTLR3 cells
• HEK-Dual™ hTLR4 cells
• HEK-Dual™ hTLR7 cells
• HEK-Dual™ hTLR8 cells
• HEK-Dual™ hTLR9 cells

Key features

• Functionally validated with a selection of PRR ligands and cytokines
• Readily assessable NF-κB or IRF activation by assessing the SEAP and Lucia luciferase activities