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NOD1 Agonist - L-Ala-gamma-D-Glu-mDAP
L-Ala-γ-D-Glu-mDAP (Tri-DAP) comprises the iE-DAP (γ-D-Glu-mDAP) dipeptide and a L-Ala residue. Tri-DAP is present in the peptidoglycan (PGN) of Gram-negative bacilli and certain Gram-positive bacteria.
Tri-DAP is recognized by the intracellular sensor NOD1, which induces a signaling cascade leading to NF-κB activation and the production of inflammatory cytokines. Tri-DAP exhibits a ~3-fold higher ability to activate NF-κB than iE-DAP.
Tri-DAP provided by InvivoGen is chemically synthesized and tested using HEK-Blue™ NOD1.
Note: Tri-DAP is a mixture of L-Ala-γ-D-Glu-D-mDAP and L- Ala-γ-D-Glu-L-mDAP.Back to the top
Specificity: NOD1 agonist
Working Concentration: 100 ng - 10 µg/ml
Endotoxin level: <0.125 EU/ml
Solubility: 10 mg/ml in water
Molecular weight: 390.39
Synonym: L-alanyl-γ-D-glutamyl-meso-diaminopimelic acidBack to the top
Tri-DAP is provided as a sterile white lyophilized powder.
- 1 mg L-Ala-γ-D-Glu-mDAP (Tri-DAP)
- 2 ml sterile endotoxin-free water
Tri-DAP is shipped at room temperature.
Store at -20°C. Upon resuspension, aliquote Tri-DAP and store at -20°C.
Product is stable 1 year at -20°C when properly stored.
Avoid repeated freeze-thaw cycles.Back to the top
L-Ala-γ-D-Glu-mDAP (Tri-DAP) comprises the iE-DAP (γ-D- Glu-mDAP) dipeptide and a L-Ala residue. Tri-DAP is present in the peptidoglycan (PGN) of a subset of bacteria that include Gram-negative bacilli and particular Gram-positive bacteria such as Bacillus subtilis and Listeria monocytogenes . Tri-DAP is recognized by NOD1 (CARD4), an intracellular sensor expressed in multiple tissues including intestinal epithelia cells. Recognition of Tri-DAP by NOD1 induces a signaling cascade involving the serine/threonine RIP2 (RICK, CARDIAK) kinase which interacts with IKK leading to the activation of NF-κB and the production of inflammatory cytokines such as TNF-α and IL-6 . Tri-DAP exhibits a ~3-fold higher ability to activate NF-κB than iE-DAP .
1. Chamaillard M. et al., 2003. An essential role for NOD1 in host recognition of bacterial peptidoglycan containing diaminopimelic acid. Nat. Immunol.4(7):702-7
2. Park JH. et al., 2007. RICK/RIP2 mediates innate immune responses induced through Nod1 and Nod2 but not TLRs. J Immunol. 178(4):2380-6.
3. Girardin SE. et al., 2003. Peptidoglycan molecular requirements allowing detection by Nod1 and Nod2.J Biol Chem. 278(43):41702-8.