Invivogen
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iE-DAP

iE-DAP Unit size Cat. code Docs Qty Price
NOD1 ligand
5 mg
tlrl-dap
+-
$124.00

NOD1 Agonist - D-gamma-Glu-mDAP

γ-D-Glu-mDAP (iE-DAP) is a dipeptide present in the peptidoglycan (PGN) of all Gram-negative and certain Gram-positive bacteria. iE-DAP is the minimal motif recognized by the intracellular receptor NOD1.

Recognition of iE-DAP by NOD1 induces NF-κB activation and the production of inflammatory cytokines. iE-DAP provided by InvivoGen is chemically synthesized and tested using HEK-Blue™ NOD1.

Note: iE-DAP is a mixture of γ-D-Glu-D-mDAP and γ-D-Glu-L-mDAP.

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Specifications

Specificity: NOD1 agonist

Working Concentration: 1 - 100 µg/ml

Endotoxin level:  <0.125 EU/ml

Solubility: 10 mg/ml in water

Formula: C12H21N3O7

Molecular weight: 319.31

Synonym: γ-D-glutamyl-meso-diaminopimelic acid

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Contents

iE-DAP is provided as a sterile white lyophilized powder.

  • 5 mg γ-D-Glu-mDAP (iE-DAP)
  • 2 ml sterile endotoxin-free water

room temperature iE-DAP is shipped at room temperature.

store Store at -20°C. Upon resuspension, aliquote iE-DAP and store at -20°C.

stable Product is stable 1 year at -20°C when properly stored.

Alert Avoid repeated freeze-thaw cycles.

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Description

γ-D-Glu-mDAP (iE-DAP) is a dipeptide present in the peptidoglycan (PGN) of a subset of bacteria that include Gram-negative bacilli and particular Gram-positive bacteria such as Bacillus subtilis and Listeria monocytogenes [1].

iE-DAP is the minimal motif recognized by NOD1 (CARD4), an intracellular sensor expressed in multiple tissues including intestinal epithelia cells. Recognition of iE-DAP by NOD1 induces a signaling cascade involving the serine/threonine RIP2 (RICK, CARDIAK) kinase which interacts with IKK leading to the activation of NF-κB and the production of inflammatory cytokines such as TNF-α and IL-6 [2].

iE-DAP provided by InvivoGen is chemically synthesized and tested using HEK-Blue™ NOD1.
Note: iE-DAP is a mixture of γ-D-Glu-D-mDAP and γ-D-Glu- L-mDAP.

 

1. Chamaillard M. et al., 2003. An essential role for NOD1 in host recognition of bacterial peptidoglycan containing diaminopimelic acid. Nat. Immunol.4(7):702-7
2. Park JH. et al., 2007. RICK/RIP2 mediates innate immune responses induced through Nod1 and Nod2 but not TLRs. J Immunol. 178(4):2380-6.

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Citations

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