iE-DAP - NOD1 Agonist

γ-D-Glu-mDAP (iE-DAP) is a dipeptide present in the peptidoglycan (PGN) of a subset of bacteria that include Gram-negative bacilli and particular Gram-positive bacteria such as Bacillus subtilis and Listeria monocytogenes [1].

iE-DAP is the minimal motif recognized by NOD1 (CARD4), an intracellular sensor expressed in multiple tissues including intestinal epithelia cells. Recognition of iE-DAP by NOD1 induces a signaling cascade involving the serine/threonine RIP2 (RICK, CARDIAK) kinase which interacts with IKK leading to the activation of NF-κB and the production of inflammatory cytokines such as TNF-α and IL-6 [2].

iE-DAP provided by InvivoGen is chemically synthesized and tested using HEK-Blue™ NOD1.
Note: iE-DAP is a mixture of γ-D-Glu-D-mDAP and γ-D-Glu- L-mDAP.

1. Chamaillard M. et al., 2003. An essential role for NOD1 in host recognition of bacterial peptidoglycan containing diaminopimelic acid. Nat. Immunol.4(7):702-7
2. Park JH. et al., 2007. RICK/RIP2 mediates innate immune responses induced through Nod1 and Nod2 but not TLRs. J Immunol. 178(4):2380-6.