iE-DAP
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Cat.code:
tlrl-dap
- Documents
ABOUT
NOD1 Agonist - D-gamma-Glu-mDAP
γ-D-Glu-mDAP (iE-DAP) is a dipeptide present in the peptidoglycan (PGN) of all Gram-negative and certain Gram-positive bacteria. iE-DAP is the minimal motif recognized by the intracellular receptor NOD1.
Recognition of iE-DAP by NOD1 induces NF-κB activation and the production of inflammatory cytokines. iE-DAP provided by InvivoGen is chemically synthesized and tested using HEK-Blue™ NOD1.
Note: iE-DAP is a mixture of γ-D-Glu-D-mDAP and γ-D-Glu-L-mDAP.
All InvivoGen products are for internal research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
C12H21N3O7
1 - 100 µg/ml
10 mg/ml in water
Activation of NOD1 in cellular assays
Each lot is functionally tested and validated.
CONTENTS
Contents
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Product:iE-DAP
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Cat code:tlrl-dap
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Quantity:5 mg
1.5 ml sterile endotoxin-free water
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
Details
γ-D-Glu-mDAP (iE-DAP) is a dipeptide present in the peptidoglycan (PGN) of a subset of bacteria that include Gram-negative bacilli and particular Gram-positive bacteria such as Bacillus subtilis and Listeria monocytogenes [1].
iE-DAP is the minimal motif recognized by NOD1 (CARD4), an intracellular sensor expressed in multiple tissues including intestinal epithelia cells. Recognition of iE-DAP by NOD1 induces a signaling cascade involving the serine/threonine RIP2 (RICK, CARDIAK) kinase which interacts with IKK leading to the activation of NF-κB and the production of inflammatory cytokines such as TNF-α and IL-6 [2].
iE-DAP provided by InvivoGen is chemically synthesized and tested using HEK-Blue™ NOD1.
Note: iE-DAP is a mixture of γ-D-Glu-D-mDAP and γ-D-Glu- L-mDAP.
1. Chamaillard M. et al., 2003. An essential role for NOD1 in host recognition of bacterial peptidoglycan containing diaminopimelic acid. Nat. Immunol.4(7):702-7
2. Park JH. et al., 2007. RICK/RIP2 mediates innate immune responses induced through Nod1 and Nod2 but not TLRs. J Immunol. 178(4):2380-6.
DOCUMENTS
Documents
Technical Data Sheet
Safety Data Sheet
Certificate of analysis
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