C12-iE-DAP

NOD1 AGONIST - ACYLATED DERIVATIVE OF iE-DAP

C12-iE-DAP is an acylated derivative of the iE-DAP dipeptide (γ-D-Glu-mDAP) present in the peptidoglycan (PGN) of Gram-negative bacilli and particular Gram-positive bacteria such as Bacillus subtilis and Listeria monocytogenes [1].

Similarly to iE-DAP, C12-iE-DAP is recognized by the intracellular sensor NOD1 (CARD4), which results in a signaling cascade involving the serine/threonine RIP2 (RICK, CARDIAK) kinase and IKK, and leading to NF-κB activation and the inflammatory cytokines production such as TNF-α and IL-6 [2].
C12-iE-DAP stimulates NOD1 at concentrations 100- to 1000-fold lower than iE-DAP.

C12-iE-DAP was generated by addition of a lauroyl (C12) group to the glutamic residue of iE-DAP. 
C12-iE-DAP provided by InvivoGen is chemically synthesized and tested using HEK-Blue™ NOD1.

Note: C12-iE-DAP is a mixture of Lauroyl-g-D-Glu-D-mDAP and Lauroyl-g-D-Glu-L-mDAP.

References:

1. Chamaillard M. et al., 2003. An essential role for NOD1 in host recognition of bacterial peptidoglycan containing diaminopimelic acid. Nat. Immunol.4(7):702-7.
2. Park JH. et al., 2007. RICK/RIP2 mediates innate immune responses induced through Nod1 and Nod2 but not TLRs. J Immunol. 178(4):2380-6.

Specifications

Specificity: NOD1 agonist

Working concentration: 1 ng - 1 μg/ml

Solubility: 10 mg/ml DMSO or methanol

Synonym: Lauroyl-γ-D-glutamyl-meso-diaminopimelic acid

Formula: C24H43N3O8

Molecular weight: 501.61

Contents

C12-iE-DAP is provided as a sterile white lyophilized powder

• 1 mg C12-iE-DAP
• 2 ml endotoxin-free water

C12-iE-DAP is shipped at room temperature.

Store at -20 ̊C. Upon resuspension, prepare aliquots of C12-iE-DAP and store at -20 ̊C.

Product is stable 1 year at -20°C when properly stored.

Avoid repeated freeze-thaw cycles.