|HEK-Blue™ mNOD1 cells||Unit size||Cat. code||Docs||Qty||Price|
murine NOD1-expressing HEK 293 cells
3-7 x 10e6 cells
Murine NOD1-expressing HEK 293 cells
HEK-Blue™-mNOD1 cells were obtained by co-transfection of the murine NOD1 gene and an optimized secreted embryonic alkaline phosphatase (SEAP) reporter gene into HEK293 cells.
The SEAP reporter gene is placed under the control of the IFN-β minimal promoter fused to five NF-κB and AP-1 binding sites. Stimulation with a NOD1 ligand activates NF-κB and AP-1 which induce the production of SEAP.
Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP.
The parental cell line of HEK-Blue™ mNOD1 cells is HEK-Blue™ Null1 cells.Back to the top
Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-freeBack to the top
- 1 vial containing 3-7 x 106 cells
- 100 μl Blasticidin (10 mg/ml)
- 100 μl Zeocin™ (100 mg/ml)
- 1 ml Normocin™ (50 mg/ml)
- 1 pouch of HEK-Blue™ Detection (cell culture medium for real-time detection of SEAP)
Shipped on dry ice (Europe, USA & Canada)Back to the top