HEK-Dual™ hTLR9
HEK-Dual™ hTLR9 (NF/IL8) | Unit size | Cat. code | Docs | Qty | Price |
---|---|---|---|---|---|
Double readout TLR cells |
3-7 x 10e6 cells |
hkd-htlr9ni |
Human TLR9 (NF-κB-SEAP / KI-[IL-8]Lucia) dual-reporter HEK293 cells
HEK-Dual™ hTLR9 (NF/IL8) cells were generated from HEK-Dual™ Null cells by stable transfection of the human TLR9 (hTLR9) gene.
Due to the knockout of TLR3 and TLR5, these cells enable the study of hTLR9 signaling without interference from other TLRs.
They respond to very low concentrations of TLR9 agonists, such as the class B CpG oligodeoxynucleotide (CpG ODN) ODN 2006. They do not respond to other TLR agonists or to the cytokine TNF-α (see validation sheet).
HEK-Dual™ hTLR9 (NF/IL8) cells are resistant to hygromycin B, Zeocin™ and blasticidin. They should be maintained in growth medium supplemented with hygromycin B and Zeocin™.
References:
1. Ohta K. et al., 2014. TLR-mediated interleukin-8 production by human submandibular gland epithelial cells. Mol Med Rep. 10(5):2377-82.
2. Roebuck KA. et al., 1999. Regulation of interleukin-8 gene expression. J Interferon Cytokine Res. 19(5):429-38.
Specifications
Antibiotic resistance: blasticidin, hygromycin, Zeocin™
Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
These cells are covered by a Limited Use License (See Terms and Conditions).
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- 1 vial containing 3-7 x 106 cells
- 1 ml of Hygromycin B Gold (100 mg/ml)
- 1 ml of Zeocin™ (100mg/ml)
- 1 ml of Normocin™ (50 mg/ml)
- 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution, a SEAP detection reagent)
- 1 pouch of QUANTI-Luc™ (Lucia luciferase detection medium)
Shipped on dry ice (Europe, USA & Canada)