HEK-Blue™ mTLR4
HEK-Blue™ mTLR4 cells | Unit size | Cat. code | Docs | Qty | Price |
---|---|---|---|---|---|
murine TLR4-expressing HEK 293 cells |
3-7 x 10e6 cells |
hkb-mtlr4 |
You may also need : HEK-Blue™ Selection | View more associated products ▼
Notification: This product is for internal research use only. Additional rights may be available. Please visit InvivoGen’s Terms and Conditions.
Murine TLR4 / NF-κB–SEAP reporter HEK293 cells
HEK-Blue™-mTLR4 cells were obtained by co-transfection of the murine TLR4, MD-2 and CD14 co-receptor genes, and an inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene into HEK293 cells.
The SEAP reporter gene is placed under the control of an IFN-β minimal promoter fused to five NF-κB and AP-1-binding sites. Stimulation with a TLR4 ligand activates NF-κB and AP-1 which induce the production of SEAP.
Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP.
The parental cell line of HEK-Blue™ mTLR4 cells is HEK-Blue™ Null1-v cells.
Back to the topSpecifications
Antibiotic resistance: blasticidin, hygromycin, Zeocin®.
Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin.
Guaranteed mycoplasma-free.
This product is covered by a Limited Use License (See Terms and Conditions).
Back to the topContents
- 1 vial containing 3-7 x 106 cells
- 2 ml 250X HEK-Blue Selection
- 1 ml Normocin™ (50 mg/ml)
- 1 pouch of HEK-Blue™ Detection (cell culture medium for real-time detection of SEAP)
Shipped on dry ice (Europe, USA, Canada and some areas in Asia)