E. coli OMVs
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Cat.code:
tlrl-omv-1
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ABOUT
E. coli outer membrane vesicles - Caspase 11-4/5 inducers
InvivoGen provides two grades of E. coli outer membrane vesicles (OMVs) to foster your research on the non‑canonical inflammasome response:
— E. coli OMVs (standard preparation) - recommended for in vitro studies
— E. coli OMVs InvivoFit™ - recommended for in vivo studies.
OMVs are small, spherical bodies secreted by gram-negative bacteria. They were recently described as potent caspase 4/5 (caspase 11 in mice) inflammasome inducers [1].
InvivoGen's OMVs have been purified from Escherichia coli BL21 using an optimized procedure, providing lot-to-lot reproducibility. Importantly, activation of the caspase 11-4/5 non-canonical inflammasome is confirmed in vivo and using THP1-HMGB1-Lucia™ cells.
Key features:
- Inducer of non-canonical caspase 11-4/5 inflammasome pathways in vitro and in vivo
- Can be used in murine or human cellular assays
- Each lot is characterized (size) and functional tested (activation of TLR2 and TLR4).
- InvivoFit™ grade: guaranteed sterile, endotoxin level >105 EU/mg
Read our review on the NLRP3 inflammasome.
Download our Practical guide on Inflammasomes.
References
1. Vanaja, S.K. et al., 2016. Bacterial Outer Membrane Vesicles Mediate Cytosolic Localization of LPS and Caspase-11 Activation. Cell 165:1106-1119.
All products are for research use only, and not for human or veterinary use.
InvivoGen also offers
InvivoFit™
InvivoFit™ is a high-quality standard specifically adapted for in vivo studies.
InvivoFit™ products are filter-sterilized (0.2 µm) and filled under strict aseptic conditions in a clean room. The level of bacterial contaminants (endotoxins and lipoproteins) in each lot is verified using a LAL assay and a TLR2 and TLR4 reporter assay
SPECIFICATIONS
Specifications
0.2 - 100 µg/mL in vitro, 10 - 100 µg/mouse in vivo
Activation of TLR2 and TLR4 by E. coli OMVs has been confirmed using HEK-Blue™ TLR cellular assays.
Inflammasome activation by E. coli OMVs has been determined by the induction of pyroptosis in THP1-HMGB1-Lucia™ cells.
Size range: 35-60 nm
Activation of TLR2 and TLR4 by E. coli OMVs has been confirmed using HEK-Blue™ TLR cellular assays.
Inflammasome activation by E. coli OMVs has been determined by the induction of pyroptosis in THP1-HMGB1-Lucia™ cells.
CONTENTS
Contents
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Product:E. coli OMVs
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Cat code:tlrl-omv-1
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Quantity:100 µg
The quantity of protein was measured by a BCA protein assay. Ligand is provided with 1.5 ml of sterile, endotoxin-free water.
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
Details
OMVs are small, non-replicative, immunogenic spherical bodies secreted by both commensal and pathogenic gram-negative bacteria [1]. They contain various pathogen-associated molecular patterns (PAMPs) such as DNA, RNA, peptidoglycan, protein, toxins and lipopolysaccharides (LPS). These PAMPs can induce both pro- and anti-inflammatory responses; a balancing act that can result in either pathogen clearance or bacterial survival [2]. They interact with pattern recognition receptors (PRRs) expressed on the surface of host cells such as Toll-like receptors (TLRs). Additionally, OMVs can be endocytosed and deliver their highly immunogenic cargo directly to the cytosol of the host cell. This triggers the non-canonical caspase 11-4/5 inflammasome in mice and humans, respectively. Ultimately, the activation of this complex results in pyroptotic cell death through the action of gasdermin D (GSDMD) and the release of pro-inflammatory cytokines such as IL-1β and IL-18 [3].
References:
1. Kulp, A. & Kuehn, M.J., 2010. Biological functions and biogenesis of secreted bacterial outer membrane vesicles. Annu Rev Microbiol 64:163-184.
2. Kaparakis-Liaskos, M. & Ferrero, R.L., 2015. Immune modulation by bacterial outer membrane vesicles. Nat Rev Immunol 15:375-387.
3. Vanaja, S.K. et al., 2016. Bacterial Outer Membrane Vesicles Mediate Cytosolic Localization of LPS and Caspase-11 Activation. Cell 165:1106-1119.
DOCUMENTS
Documents
Technical Data Sheet
Validation Data Sheet
Safety Data Sheet
Certificate of analysis
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