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Nano-SiO2

Nano-SiO2 Unit size Cat. code Docs Qty Price
SiO2; Silicon Dioxid Nanoparticles
20 mg (2 x 10 mg)
tlrl-sio-2
+-
$224.00

You may also need : NLRP3 KO & KD THP-1 Cells | View more associated products

Inflammasome activation with Nano-SiO<sub>2</sub>
Inflammasome activation with Nano-SiO2

NLRP3 Inflammasome Inducer - Silicon Dioxid Nanoparticles

SiO2 nanoparticles (Nano-SiO2) are single particles of silica dioxide, an inorganic metal oxide, with a diameter of less than 100 nm.
Several studies have demonstrated that Nano-SiO2 trigger the NLRP3 inflammasome-dependent induction of interleukin-1β (IL-1β) and IL-18 in vitro and in vivo [1,2].

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The biological activity of Nano-SiO2  has been validated using InvivoGen's THP-1 Null cells and HEK-Blue™ IL-1β cells.

Key features:

  • Potent inducer of the NLRP3 inflammasome
  • Nanoparticles with a diameter of <100 nm
  • Each lot is functionally tested

 

Read our review on the NLRP3 inflammasome.

Download our Practical guide on Inflammasomes.

 

References:

1. Nakayama M. et al., 2018. Macrophage recognition of crystals and nanoparticles. Front Immunol. 9:103. 
2. He Y. et al., 2016. NEK7 is an essential mediator of NLRP3 activation downstream of potassium efflux. Nature. 530(7590):354-7.

Figures

Evaluation of NLRP3 inflammasome activation with Nano-SiO2
Evaluation of NLRP3 inflammasome activation with Nano-SiO2

IL-1β production in THP1-Null cells.

THP1-Null cells, primed with LPS (1 µg/ml for 3h), were stimulated with increasing concentrations of Nano-SiO2. After overnight incubation,  IL-1β secretion was analyzed by adding 50  µl of supernatant from treated THP1-Null cells to HEK-Blue™ IL-1β cells. IL-1β-induced activation of NF-κB was assessed by measuring the levels of SEAP in the supernatant of HEK-Blue™ IL-1β cells using QUANTI-Blue™ Solution, and by reading the optical density (OD) at 630 nm.

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Specifications

Working concentration: 10-250 µg/ml

CAS number: 7631-86-9

Linear formula: SiO2

Molecular weight: 60.08 g/mol

Quality control:

  • The biological activity has been validated using cellular assays.
  • The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.
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Contents

  • 20 mg (2 x 10 mg) Nano-SiO2 (nanoparticles of silica dioxide)

room temperature Nano-SiO2 is shipped at room temperature.

store Store at room temperature.

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Details

The NLRP3 inflammasome is an intracellular multi-protein complex that plays a central role in innate immunity. It is activated by a two-step process. A first signal (‘priming’) is provided by pathogen-associated molecular patterns (PAMPs) or cytokines. It allows the transcriptional upregulation of key inflammasome actors and the post-translational modification of NLRP3 . The second signal (‘activation’) is provided by a wide array of stimuli including microbial toxins, endogenous molecules or crystalline substances. The current paradigm is that NLRP3 does not bind directly to these molecules. Rather it senses downstream cytosolic stress signals such as K+ efflux. This  triggers inflammasome multimerization and pro-caspase-1 maturation. Proximity-induced autolytic activation of caspase-1 leads to the formation of gasdermin D (GSDMD) pores at the cell surface, allowing IL-1β/IL-18 and alarmin secretion, and ultimately, pyroptosis [1,2].

 

References:

1. Swanson K.V. et al., 2019. The NLRP3 inflammasome: molecular activation and regulation to therapeutics. Nat. Rev. Immunol. 19:477.
2. Groslambert M. & Py B. 2018. Spotlight on the NLRP3 inflammasome pathway. J. Inflamm. Res. 11:359.

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Citations

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