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Dectin-1 Agonist - Beta-glucan from Lasallia pustulata
Beta-glucan from Lasallia pustulata - β(1➝6)-glucan
Pustulan is a median molecular weight (20 kDa), linear (1-6) linked β-D-glucan from lichen Lasallia pustulata.
Pustulan is recognized by the membrane bound Dectin-1, a C-type lectin-like pattern recognition receptor .
Detection of β-glucans by Dectin-1 receptor leads to the CARD9-dependent activation of NF-κB and MAP kinases.
Studies have shown that pustulan can stimulate innate immune responses, inducing heat shock protein expression, eliciting phagocytosis , and production of proinflammatory cytokines .
1. Willment Ja. et al., 2001. Characterization of the human beta -glucan receptor and its alternatively spliced isoforms. J Biol Chem. 276(47):43818- 23.
2. Goodridge HS. et al., 2009. Beta-glucan recognition by the innate immune system. Immunol Rev. 230(1):38-50.
3. Iubin-Bejerano I. et al., 2007. Phagocytosis by human neutrophils is stimulated by a unique fungal cell wall component. Cell Host Microbe. 2(1):55-67.
4. Noss I. et al., 2013. Comparison of the potency of a variety of β-glucans to induce cytokine production in human whole blood. Innate Immun. 19(1):10-9.
Specificity: Dectin-1 ligand
CAS number: 37331-28-5
Partial structure: β(1➝6)-glucan
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- 100 mg pustulan provided as a lyophilized powder
Pustulan is shipped at room temperature.
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Responses to β-glucans.
RAW-Blue™ cells and HEK-Blue™ mDectin-1 cells, which express the mouse dectin-1a gene, were stimulated with 100 µg/ml of various β-glucans.
After 24h incubation, NF-κB activation was assessed by measuring the levels of SEAP (secreted embryonic alkaline phosphatase) using the QUANTI-Blue™ assay.
Stimulatory activity of Dectin-1 ligands:
HEK-Blue™ hDectin-1a and HEK-Blue™ hDectin-1b cells were stimulated with 1 µg/ml or 10 µg/ml Dectin-1 ligands, respectively, and 10 µg/ml TDB.
After 24h incubation, Dectin-1-induced NF-κB activation was assessed by measuring the levels of SEAP using the QUANTI-Blue™ assay.