Scleroglucan - Dectin-1 Agonist

Beta-glucan from Sclerotium rolfsii - β(1➝3, 1➝6)-glucan

Scleroglucan is a high molecular weight (>1000 kDa) polysaccharide produced by fermentation of the filamentous fungus Sclerotium rolfsii. It consists of a linear β(1-3) D-glucose backbone with one β(1-6) D-glucose side chain every three main residues.

Scleroglucan is recognized by Dectin-1 [1] and strongly activates HEK-Blue™ Dectin-1 and RAW-Blue™ cells. Detection of β-glucans by Dectin-1 receptor leads to the CARD9-dependent activation of NF-κB and MAP kinases [2].

Similarly to other β-glucans such as schizoplyllan, another high molecular weight β(1->3, 1->6)-glucan, scleroglucan is recognized by Dectin-1 and strongly activates NF-κB (see figure 1 below).

References:

1. Adams EL. et al., 2008. Differential high-affinity interaction of dectin-1 with natural or synthetic glucans is dependent upon primary structure and is influenced by polymer chain length and side-chain branching. J Pharmacol Exp Ther. 325(1):115-23
2. Goodridge HS. et al., 2009. Beta-glucan recognition by the innate immune system. Immunol Rev. 230(1):38-50.