HEK-Blue™ mTLR3
| HEK-Blue™ mTLR3 cells | Unit size | Cat. code | Docs | Qty | Price |
|---|---|---|---|---|---|
murine TLR3-expressing HEK 293 cells |
3-7 x 10e6 cells |
hkb-mtlr3 |
Murine TLR3 / NF-κB–SEAP reporter HEK293 cells
HEK-Blue™-mTLR3 cells were obtained by co-transfection of the murine TLR3 gene and an inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene into HEK293 cells.
The SEAP gene is placed under the control of the IFN-β minimal promoter fused to five NF-κB and AP-1-binding sites. Stimulation with a TLR3 ligand activates NF-κB and AP-1 which induce the production of SEAP.
Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP.
The parental cell line of HEK-Blue™ mTLR3 cells is HEK-Blue™ Null1-k cells.
Figures
Specifications
Antibiotic resistance: blasticidin, Zeocin®.
Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™.
Guaranteed mycoplasma-free.
This product is covered by a Limited Use License (See Terms and Conditions).
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- 1 vial containing 3-7 x 106 cells
- 100 μl Blasticidin (10 mg/ml)
- 100 μl Zeocin® (100 mg/ml)
- 1 ml Normocin™ (50 mg/ml)
- 1 pouch of HEK-Blue™ Detection (cell culture medium for real-time detection of SEAP)
Shipped on dry ice (Europe, USA, Canada and some areas in Asia)
Any questions about our cell lines ?