Poly(A:U)

TLR3 agonist - Polyadenylic-polyuridylic acid

Polyadenylic–polyuridylic acid (poly(A:U)) is a synthetic double stranded RNA molecule that signals only through TLR3.

Recognition of poly(A:U) by TLR3 induces the activation of dendritic cells and T lymphocytes.

When combined with an antigen in mice, poly(A:U) has been shown to promote antigen-specific Th1-immune responses and boost antibody production [1].

The potent adjuvant activity of poly(A:U) has been exploited in the treatment of breast cancers that express TLR3 [2].

References:

1. Wang L. et al., 2002. Noncoding RNA danger motifs bridge innate and adaptive immunity and are potent adjuvants for vaccination. J Clin Invest 110:1175–84.
2. Conforti R. et al., 2010. Opposing effects of toll-like receptor (TLR3) signaling in tumors can be therapeutically uncoupled to optimize the anticancer efficacy of TLR3 ligands. Cancer Res. 70(2):490-500.

Specifications

Specificity: TLR3 agonist

Working Concentration: 300 ng - 100 μg/ml

CAS number: 24936-38-7

Solubility: 1 mg/ml in physiological water (NaCl 0.9%) 

Contents

Poly(A:U) is provided lyophilized.

• 10 mg sterile lyophilized poly(A:U).
• 10 ml sterile physiological water.

Poly(A:U) is shipped at room temperature.

Upon a receip it should be stored at 4°C.

Upon resuspension, prepare aliquots of Poly(A:U) and store at 4°C or -20°C.

Lyophilized product is stable for 1 year at 4°C when properly stored.

Resuspended product is stable for 1 month at 4°C and for 1 year at -20°C.

Avoid repeated freeze-thaw cycles.