|Poly(I:C) (LMW)||Unit size||Cat. code||Docs||Qty||Price|
Short synthetic analog of dsRNA
TLR3 agonist - Polyinosine-polycytidylic acid Low molecular weight
Polyinosinic-polycytidylic acid (usually abbreviated as poly(I:C) or poly(rI):poly(rC)) is a synthetic analog of double-stranded RNA (dsRNA), pathogen-associated molecular pattern (PAMP) associated with viral infection. Poly(I:C) is recognized by the antiviral pattern recognition receptors TLR3, RIG-I/MDA5 and PKR, thereby inducing signaling via multiple inflammatory pathways, including NF-kB and IRF.
Low Molecular Weight Poly(I:C) comprises short strands of inosine poly(I) homopolymer annealed to strands of cytidine poly(C) homopolymer. The average size of Poly(I:C) LMW is from 0.2 kb to 1 kb.
Poly(I:C) LMW is functionally validated for TLR3 potency using HEK-Blue™ TLR3 Cells. It is tested in TLR2 and TLR4 cellular assays to confirm the absence of microbial contaminants (e.g. endotoxin or bacterial-membrane components).
CAS number: 31852-29-6
Working concentration: 30 ng- 10 μg/ml
Solubility: 20 mg/ml in physiological water (NaCl 0.9%)Back to the top
• Poly(I:C)-LMW is provided lyophilized and is available in two quantities:
- 25 mg: (catalog # tlrl-picw)
- 250 mg: (catalog # tlrl-picw-250)
• Sterile endotoxin-free physiological water (NaCl 0.9%), 10 ml is provided with catalog #tlrl-picw and 2 x 25 ml with catalog #tlrl-picw-250
Poly(I:C)-LMW is shipped at room temperature and should be stored at 4°C. Upon resuspension, prepare aliquots of Poly(I:C)-LMW and store at -20°C for long term storage. Store at 4°C for short term storage. Lyophilized product is stable 1 year at 4°C when properly stored. Resuspended product is stable 1 month at 4°C and 1 year at -20°C. Avoid repeated freeze-thaw cycles.Back to the top
Polyinosinic-polycytidylic acid (poly(I:C)) is a synthetic analog of double stranded RNA (dsRNA), a molecular pattern associated with viral infection.
Both natural and synthetic dsRNAs are known to induce type I interferons (IFN) and other cytokines production.
Poly(I:C) is recognized by Toll-like receptor 3 (TLR3) [1, 2]. Upon poly(I:C) recognition, TLR3 activates the transcription factor interferon regulatory factor 3 (IRF3), through the adapter protein Toll-IL-1 receptor (TIR) domain-containing adapter inducing IFN-β (TRIF, also known as TICAM-1) . Activation of IRF3 leads to the production of type I IFNs, especially IFN-β.
A second pathway involves the recruitment of TNF receptor-associated factor 6 (TRAF6) or receptor interacting protein 1 (RIP1), with the subsequent activation of the transcription factors NF-κB and AP-1 . Activation of this pathway triggers the production of inflammatory cytokines and chemokines such as TNF-α, IL-6 and CXCL10.
Poly(I:C) is also recognized by the cytosolic RNA helicases retinoic acid-inducible protein I (RIG-I) and melanoma differentiation-associate gene 5 (MDA-5) .
1. Alexopoulou L. et al., 2001. Recognition of double-stranded RNA and activation of NFkappaB by Toll-like receptor 3. Nature, 413(6857):732-8.
2. Matsumoto M. et al., 2002. Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double- stranded RNA-mediated signaling. BBRC, 293(5):1364-9.
3. Yamamoto M. et al., 2003. Role of Adaptor TRIF in the MyD88-Independent Toll-Like Receptor Signaling Pathway. Science 301: 640.
4. Kawai T & Akira S., 2008. Tolllikereceptor and RIG-I-like receptor signaling. Ann N Y Acad Sci. 1143:1-20. Review.
5. Kato H. et al., 2006. Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses. Nature. 441(7089):101-5.