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Poly(I:C) (HMW) Biotin

Poly(I:C) (HMW) Biotin Unit size Cat. code Docs Qty Price
Biotin labeled TLR3 agonist
10 µg
tlrl-picb
+-
$150.00

Biotin-labeled synthetic analog of dsRNA

Poly(I:C) HMW Biotin was chemically labeled by covalent coupling of biotin with poly(I:C) HMW (High molecular weight).

Polyinosinic-polycytidylic acid (poly(I:C)) is a synthetic analog of double stranded RNA (dsRNA), a molecular pattern associated with viral infection.

Both natural and synthetic dsRNAs are known to induce type I interferons (IFN) and other cytokines production.

Poly(I:C) is recognized by Toll-like receptor 3 (TLR3) [1, 2].

 

1. Alexopoulou L. et al., 2001. Recognition of double-stranded RNA and activation of NF-kB by Toll-like receptor 3. Nature, 413:732-8.
2. Matsumoto M. et al., 2002. Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling. BBRC 293:1364-9.

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Specifications

Specificity: TLR3 agonist

Working Concentration: 30 ng - 10 μg/ml

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Contents

Poly(I:C) HMW Biotin is provided lyophilized:

  • 10 µg Poly(I:C) HMW Biotin
  • 1.5 ml endotoxin-free water

Poly(I:C) HMW Biotin is shipped at room temperature

Upon receipt it should be stored at -20°C for up to 6 months.

Upon resuspension, Poly(I:C) HMW Biotin should be aliquoted and stored at -20˚C.

Resuspended product is stable 3 months at -20°C.

Avoid repeated freeze-thaw cycles.

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Description

TLR3 activation with Poly(I:C) HMW Biotin

Poly(I:C) HMW Biotin-induced TLR3 activation can be monitored using TLR3 reporter cell lines, such as HEK-Blue™ hTLR3 cells.

HEK-Blue™ hTLR3 cells were transfected with the human TLR3 gene and an NF-kB/AP-1-inducible SEAP (secreted alkaline phosphatase) reporter gene.

Levels of SEAP can be easily determined with QUANTI-Blue™ (a detection medium that turns purple/blue in the presence of alkaline phosphatase).

Description

Poly(I:C) HMW Biotin was chemically labeled by covalent coupling of biotin with poly(I:C) HMW (High molecular weight).

Polyinosinic-polycytidylic acid (poly(I:C)) is a synthetic analog of double stranded RNA (dsRNA), a molecular pattern associated with viral infection.

Both natural and synthetic dsRNAs are known to induce type I interferons (IFN) and other cytokines production. Poly(I:C) is recognized by Toll-like receptor 3 (TLR3) [1, 2].

Upon poly(I:C) recognition, TLR3 activates the transcription factor interferon regulatory factor 3 (IRF3), through the adapter protein Toll-IL-1 receptor (TIR) domain-containing adapter inducing IFN-β (TRIF, also known as TICAM-1) [3].

Activation of IRF3 leads to the production of type I IFNs, especially IFN-β. A second pathway involves the recruitment of TNF receptor-associated factor 6 (TRAF6) or receptor interacting protein 1 (RIP1), with the subsequent activation of the transcription factors NF-κB and AP-1 [4].

Activation of this pathway triggers the production of inflammatory cytokines and chemokines such as TNF-α, IL-6 and CXCL10. Poly(I:C) is also recognized by the cytosolic RNA helicases retinoic acid-inducible protein I (RIG-I) and melanoma differentiation-associate gene 5 (MDA-5) [5].

 

1. Alexopoulou L. et al., 2001. Recognition of double-stranded RNA and activation of NF-kB by Toll-like receptor 3. Nature, 413:732-8.
2. Matsumoto M. et al., 2002. Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling. BBRC 293:1364-9.
3. Yamamoto M. et al., 2003. Cutting edge: A novel Toll/Il-1 receptor domain-containing adapter that preferentially activates the IFN-β promoter in the Toll-like receptor signaling. Science 301:640.
4. Kawai T. & Akira S., 2008. Toll-like receptor and RIG-I-like receptor signaling. Ann N Y Acad Sci. 1143:1-20.
5. Kato H. et al., 2006. Small interfering RNAs mediate sequence-independent gene suppression and induce immune activation by signaling through toll-like receptor 3. Nature 441:101-5.

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