|HEK-Blue™ hMD2-CD14 cells||Unit size||Cat. code||Docs||Qty||Price|
human MD2-CD14-expressing HEK 293 cells
3-7 x 10e6 cells
HEK-Blue™ MD2-CD14 cells were obtained by co-transfection of the human MD-2 and CD14 genes and an inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene into HEK293 cells.
The SEAP reporter gene is under the control of the IL-12 p40 promoter fused to five NF-κB and AP-1 binding sites.
Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP.
The parental cell line of HEK-Blue™ MD2-CD14 cells is HEK-Blue™ Null2 cells.
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™.
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