HEK-Blue™ hMD2-CD14

HEK-Blue™ hMD2-CD14 cells Unit size Cat. code Docs Qty Price
human MD2-CD14-expressing HEK 293 cells
3-7 x 10e6 cells

Human MD2-CD14 / NF-κB / SEAP reporter HEK293 cells

HEK-Blue™ MD2-CD14 cells were obtained by co-transfection of the human MD-2 and CD14 genes and an inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene into HEK293 cells.

The SEAP reporter gene is under the control of the IL-12 p40 promoter fused to five NF-κB and AP-1 binding sites.

Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP.

The parental cell line of HEK-Blue™ MD2-CD14 cells is HEK-Blue™ Null2 cells.

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Antibiotic resistance: hygromycin, Zeocin™.

Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™.

Guaranteed mycoplasma-free.


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Shipped on dry ice

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Visit our FAQ Any questions about our cell lines ? Visit our frequently asked questions page

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