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LPS-EB (LPS from E. coli O111:B4)

LPS-EB Unit size Cat. code Docs Qty Price
Standard LPS, E. coli 0111:B4
5 mg
tlrl-eblps
+-
$135.00
LPS-EB Ultrapure Unit size Cat. code Docs Qty Price
Ultrapure LPS, E. coli 0111:B4
5 x 10e6 EU
tlrl-3pelps
+-
$183.00

Lipopolysaccharide from E. coli O111:B4

InvivoGen provides LPS-EB with two grades of purity:

  • LPS-EB is a standard lipopolysaccharide (LPS) preparation. It was extracted by a phenol-water mixture.

LPS-EB contains other bacterial components, such as lipopeptides, and therefore stimulates both TLR4 and TLR2.

  • Ultra-pure LPS-EB was extracted by successive enzymatic hydrolysis steps and purified by the phenol-TEA-DOC extraction protocol. 

Ultrapure LPS-EB only activates the TLR4 pathway.

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Specifications

LPS-EB

Specificity: TLR4 and TLR2 agonist.

Solubility: 5 mg/ml in water.

Working concentration: 10 ng - 10 µg/ml.

LPS-EB Ultrapure

Specificity: TLR4 agonist.

Solubility: 5  x 10e6 EU/ml in water.

Working concentration: 10e1 - 10e4 EU/ml.

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Contents

LPS-EB

  • 5 mg lipopolysaccharide from E. coli 0111:B4 (LPS-EB ).
  • 1.5 ml endotoxin-free water.

LPS-EB is shipped at room temperature.

Upon receipt it should be stored at 4°C.

Resuspended LPS-EB may be stored at 4 ̊C for 1 month.

 

LPS-EB Ultrapure

  • 5 x 106 EU  ultrapure lipopolysaccharide from E. coli 0111:B4 (LPS-EB Ultrapure).
  • 1.5 ml endotoxin-free water.

LPS-EB Ultrapure is shipped at room temperature.

Upon receipt it should be stored at -20 ̊C.

Lyophilized product is stable 1 year at -20°C.

Upon resuspension, prepare aliquots of LPS-EB Ultrapure and store at 4°C for short term storage or -20 ̊C for long term storage.

Resuspended product is stable 1 month at 4°C and 6 months at -20°C.

Avoid repeated freeze-thaw cycles.

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Description

LPS-EB is lipopolysaccharide from E. coli 0111:B4. LPS is the principal component of Gram negative bacteria that activates the innate immune system.

LPS recognition is predominantly mediated by TLR4 [1]. This recognition involves the binding of LPS with lipopolysaccharide-binding protein (LBP) and subsequently with CD14 which physically associates with a complex including TLR4 and MD2 [2].

Formation of the TLR4-centered LPS receptor complex induces the production of proinflammatory cytokines through the MyD88 pathway.

LPS signaling also involves a MyD88- independent cascade that mediates the expression of IFN-inducible genes.

Most LPS preparations on the market are contaminated by other bacterial components, such as lipoproteins, thus activating TLR2 signaling as well as TLR4 signaling. High concentrations (1 µg/ml) of the standard preparation of LPS-EB can induce TLR2 activity, whereas LPS-EB Ultrapure only activates the TLR4 pathway..

1. Poltorak A. et al., 1998. Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in TLR4 gene. Science, 282(5396): 2085-8.
2. Shimazu R. et al., 1999. MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4. J Exp Med, 189(11):1777-82.
3. Netea MG. et al., 2002. Does the shape of lipid A determine the interaction of LPS with Toll-like receptors? Trends Immunol, 23(3):135-9.

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Citations

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