MPLA Synthetic - TLR4 Agonist

Synthetic monophosphoryl lipid A

ABOUT

TLR4 Agonist - Synthetic monophosphoryl lipid A

Synthetic lipid A from E. coli serotype R515 (MPLAs) is a monophosphoryl lipid A with 6 fatty acyl groups. It is structurally very similar to natural MPLA except that natural MPLA contains a mixture of 5, 6, and 7 acyl lipid A. The number of fatty acids is a major determinant of the immunogenicity of endotoxin.

Synthetic E. coli lipid A activates TLR4 but does not activate TLR2 even at high concentrations, reflecting its high purity. This product can be used for immunomodulatory studies as it is a pure monophosphoryl lipid A containing 6 fatty acyl groups. 

InvivoGen also offers MPLA Synthetic VacciGrade™, a high-quality pre-clinical grade suited for in vivo studies.

 

Background

Monophosphoryl Lipid A (MPLA) is a low-toxicity derivative of lipopolysaccharide (LPS) that retains the immunologically active lipid A portion of the parent molecule. Both LPS and MPLA are TLR4 agonists, but they signal through different adaptors, MyD88 and TRIF, respectively1, 2. The reduced toxicity of MPLA is attributed to the preferential recruitment of TRIF upon TLR4 activation, resulting in decreased induction of inflammatory cytokines. MPLA is widely used as a vaccine adjuvant due to its potent immunomodulatory properties and low inflammatory toxicity1, 2

 

1. Sastry M. et al., 2017. Adjuvants and the vaccine response to the DS-Cav1stabilized fusion glycoprotein of respiratory syncytial virus. PLoS One. 12(10):e0186854.
2. Cui W. et al., 2014. TLR4 ligands lipopolysaccharide and monophosphoryl lipid a differentially regulate effector and memory CD8+ T Cell differentiation. J Immunol. 192(9):4221-32.
3. Steimle A. et al., 2017. Structure and function: Lipid A modifications in commensals and pathogens. Int J Med Microbiol. 306(5):290-301.

All products are for internal research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Source
Synthetic
Target

TLR4

CAS number
1246298-63-4
Chemical formula

C96H184N3O22P

Molecular weight
1763.47 g/mol
Working concentration

300 pg/ml - 100 ng/ml

Solubility

1 mg/ml in DMSO

Appearance (form)
Clear lipidic film
Appearance (color)
Colorless
Reconstitution buffer
DMSO (not provided)
Tested applications

TLR4 activation

Applications

TLR4 activation

Quality control

Each lot is functionally tested and validated.

Additional information

The absence of bacterial contamination (e.g. lipoproteins) has been confirmed using HEK-Blue™ TLR2 cells.

CONTENTS

Contents

  • Product: 
    MPLAs
  • Cat code: 
    tlrl-mpls
  • Quantity: 
    1 mg

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C
    Stability: -20°C for up to 6 months

    Caution:

    • Avoid repeated freeze-thaw cycles

DOCUMENTS

Documents

MPLAs

Technical Data Sheet

Safety Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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