|LPS-EB Biotin||Unit size||Cat. code||Docs||Qty||Price|
Biotinylated TLR4 Agonist
Biotinylated LPS from Escherichia coli 0111:B4
LPS is the principal component of Gram-negative bacteria that is recognized predominantly by Toll-like receptor 4 (TLR4). Ultimately, the recognition of LPS leads to the activation of NF-κB and the production of proinflammatory cytokines . LPS-EB Biotin is a biotinylated ultrapure smooth (S)-form LPS purified from the Gram-negative bacteria E. coli 0111:B4. Biotin has been bound to the oxidized carbohydrates of LPS-EB using biotin-LC-hydrazide.
LPS-EB Biotin can be used for the detection of LPS binding sites on target cells by flow cytometry and/or fluorescence microscopy.
InvivoGen provides LPS-EB Biotin as an Ultrapure grade of purity:
LPS-EB Biotin is extracted by successive enzymatic hydrolysis steps and purified by the previously described phenol-TEA-DOC extraction protocol . This process removes contaminating lipoproteins and therefore LPS-EB Biotin only activates TLR4.
1. Kuzmich, N.N. et al., 2017. TLR4 Signaling Pathway Modulators as Potential Therapeutics in Inflammation and Sepsis. Vaccines (Basel) 5unol. 165(2):618-22.
2. Hirschfeld M. et al., 2000. Cutting edge: repurification of lipopolysaccharide eliminates signaling through both human and murine toll-like receptor 2. J Immunol. 165(2):618-22.
Specificity: TLR4 agonist
Working concentration: 10 ng - 10 μg/ml
Endotoxin level: ≥1 x 105 EU/mg (measurement with HEK-Blue™ LPS Detection Kit 2)Back to the top
- 500 μg biotinylated ultrapure E. coli O111:B4 LPS (LPS-EB Biotin)
- 1.5 ml endotoxin-free water
LPS-EB Biotin is shipped at room temperature
Stored at -20°C.
Resuspended biotinylated LPS may be stored at 4°C for 1 month.Back to the top