LPS-EB Biotin - TLR4 Agonist

Biotinylated ultrapure LPS from E. coli 0111:B4

ABOUT

Ultrapure biotinylated LPS from Escherichia coli 0111:B4

LPS-EB Biotin is biotinylated ultrapure smooth lipopolysaccharide (sLPS) from the Gram-negative bacteria E. coli  0111:B4.

LPS is recognized by toll-like receptor 4 (TLR4), which interacts with three different extracellular proteins: LPS binding protein (LBP), CD14, and myeloid differentiation protein 2 (MD-2), to induce a signaling cascade leading to the activation of NF-κB and the production of proinflammatory cytokines [1, 2]. 

Most LPS preparations on the market are contaminated by other bacterial components, such as lipoproteins, thus activating TLR2 signaling as well as TLR4 signaling. The ultrapure preparation that InvivoGen provides only activates the TLR4 pathway.

In this product, biotin has been bound to LPS’s oxidized carbohydrates using biotin-LC-hydrazide as previously described [3]. The labeled ligand retains the biological activity of LPS. LPS‑EB Biotin can be used for the detection of LPS binding sites by cytometry or fluorescence microscopy.  

 

Key features of LPS-EB Biotin:

  • Highly specific and potent TLR4 agonist
  • Ultrapure grade of LPS that only activates TLR4
  • Each lot is functionally tested

     

References:

1. Kuzmich, N.N. et al., 2017. TLR4 signaling pathway modulators as potential therapeutics in inflammation and sepsis. Vaccines (Basel) 5(4):34.
2. Hirschfeld M. et al., 2000. Cutting edge: repurification of lipopolysaccharide eliminates signaling through both human and murine toll-like receptor 2. J Immunol. 165(2):618-22.
3. Luk J.M. et al., 1995. Biotinylated lipopolysaccharide binds to endotoxin receptor in endothelial and monocytic cells. Anal Biochem 232:217.

 

Learn more about TLR4 and its co-receptors

All InvivoGen products are for internal research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Source
E. coli O111:B4
Target

TLR4

Working concentration

10 ng/ml - 10 μg/ml

Solubility

1 mg/ml in water

Appearance (form)
Lyophilized
Appearance (color)
White
Reconstitution buffer
Endotoxin-free water (provided)
Endotoxin

≥ 1 x 105 EU/mg (measurement by kinetic chromogenic LAL assay)

Tested applications

TLR4 activation in a cellular assay 
Detection of LPS binding sites using flow cytometry

Applications

TLR4 activation

Ligand-binding studies

Flow cytometry

Fluorescent microscopy

Quality control

Each lot is functionally tested and validated using cellular assays.

Additional information

The absence of bacterial contamination (e.g. lipoproteins) has been confirmed using HEK‑Blue™ hTLR2 cellular assays.

CONTENTS

Contents

  • Product: 
    LPS-EB Biotin
  • Cat code: 
    tlrl-lpsbiot
  • Quantity: 
    500 µg
Includes:

1.5 ml endotoxin-free water

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C

    Caution:

    • Avoid repeated freeze-thaw cycles

DOCUMENTS

Documents

LPS-EB Biotin

Technical Data Sheet

Safety Data Sheet

Validation Data Sheet

Certificate of analysis

Need a CoA ?

CUSTOMER SERVICE & TECHNICAL SUPPORT

Question about this product ?