LPS-EB Biotin
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Cat.code:
tlrl-lpsbiot
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ABOUT
Biotinylated LPS from Escherichia coli 0111:B4
LPS-EB Biotin is biotinylated ultrapure smooth lipopolysaccharide (sLPS) from the Gram-negative bacteria E. coli 0111:B4.
LPS is recognized by toll-like receptor 4 (TLR4) which interacts with three different extracellular proteins: LPS binding protein (LBP), CD14, and, myeloid differentiation protein 2 (MD-2), to induce a signaling cascade leading to the activation of NF-κB and the production of proinflammatory cytokines [1, 2].
Most LPS preparations on the market are contaminated by other bacterial components, such as lipoproteins, thus activating TLR2 signaling as well as TLR4 signaling. The ultrapure preparation that InvivoGen provides only activates the TLR4 pathway.
In this product, biotin has been bound to LPS’s oxidized carbohydrates using biotin-LC-hydrazide as previously described [3]. The labeled ligand retains the biological activity of LPS. LPS‑EB Biotin can be used for the detection of LPS binding sites by cytometry or fluorescence microscopy.
Key features of LPS-EB Biotin:
- Highly specific and potent TLR4 agonist
- Ultrapure grade of LPS that only activates TLR4
Each lot is functionally tested
References:
1. Kuzmich, N.N. et al., 2017. TLR4 signaling pathway modulators as potential therapeutics in inflammation and sepsis. Vaccines (Basel) 5(4):34.
2. Hirschfeld M. et al., 2000. Cutting edge: repurification of lipopolysaccharide eliminates signaling through both human and murine toll-like receptor 2. J Immunol. 165(2):618-22.
3. Luk J.M. et al., 1995. Biotinylated lipopolysaccharide binds to endotoxin receptor in endothelial and monocytic cells. Anal Biochem 232:217.
Learn more about TLR4 and its co-receptors
All InvivoGen products are for internal research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
10 ng/ml to 10 μg/ml
TLR4 activation in cellular assay, detection of LPS binding sites by flow cytometry
Activation of TLR4 has been confirmed using HEK-Blue™ TLR4 cells, Absence of bacterial components (e.g. lipoproteins) has been tested for in HEK-Blue™ TLR2 cells, Biotin coupling has been validated by flow cytometry.
CONTENTS
Contents
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Product:LPS-EB Biotin
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Cat code:tlrl-lpsbiot
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Quantity:500 µg
1.5 ml endotoxin-free water
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
DOCUMENTS
Documents
Technical Data Sheet
Safety Data Sheet
Validation Data Sheet
Certificate of analysis
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