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LPS-EB Biotin

LPS-EB Biotin Unit size Cat. code Docs Qty Price
Biotinylated TLR4 Agonist
500 µg
tlrl-3blps
+-
$177.00

November 15th2018 - SPECIFICATION UPDATE NOTIFICATION
We now use the HEK-Blue™ LPS Detection Kit 2 to assess the endotoxin levels in this product. Therefore, the specification has been changed from
5x105 EU/mg (with the kinetic chromogenic LAL assay) to 1x105 EU/mg.

Biotinylated LPS from Escherichia coli 0111:B4

LPS is the principal component of Gram-negative bacteria that is recognized predominantly by Toll-like receptor 4 (TLR4). Ultimately, the recognition of LPS leads to the activation of NF-κB and the production of proinflammatory cytokines [1]. LPS-EB Biotin is a biotinylated ultrapure smooth (S)-form LPS purified from the Gram-negative bacteria E. coli 0111:B4. Biotin has been bound to the oxidized carbohydrates of LPS-EB using biotin-LC-hydrazide.

LPS-EB Biotin can be used for the detection of LPS binding sites on target cells by flow cytometry and/or fluorescence microscopy.

 

InvivoGen provides LPS-EB Biotin as an Ultrapure grade of purity:

  • LPS-EB Biotin is extracted by successive enzymatic hydrolysis steps and purified by the previously described phenol-TEA-DOC extraction protocol [2]. This process removes contaminating lipoproteins and therefore LPS-EB Biotin only activates TLR4.
     

References:

1. Kuzmich, N.N. et al., 2017. TLR4 Signaling Pathway Modulators as Potential Therapeutics in Inflammation and Sepsis. Vaccines (Basel) 5unol. 165(2):618-22.
2. Hirschfeld M. et al., 2000. Cutting edge: repurification of lipopolysaccharide eliminates signaling through both human and murine toll-like receptor 2. J Immunol. 165(2):618-22.

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Specifications

Specificity: TLR4 agonist

Working concentration: 10 ng - 10 μg/ml

Endotoxin level:  ≥1 x 105 EU/mg (measurement with HEK-Blue™  LPS Detection Kit 2)

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Contents

  • 500 μg biotinylated ultrapure E. coli O111:B4 LPS (LPS-EB Biotin)
  • 1.5 ml endotoxin-free water

room temperature LPS-EB Biotin is shipped at room temperature

store Stored at -20°C.

Resuspended biotinylated LPS may be stored at 4°C for 1 month.

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Citations

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