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LPS-B5 (LPS from E. coli 055:B5)

LPS-B5 (Standard) Unit size Cat. code Docs Qty Price
TLR2/TLR4 Agonist - LPS from E. coli 055:B5
5 mg
tlrl-b5lps
+-
$135.00
LPS-B5 Ultrapure Unit size Cat. code Docs Qty Price
TLR4 Agonist - Ultrapure LPS from E. coli 055:B5
5 mg
tlrl-pb5lps
+-
$183.00

Lipopolysaccharide from Escherichia coli 055:B5

LPS-B5 is a preparation of a smooth (S)-form lipopolysaccharide (LPS) from the Gram-negative bacteria E. coli 055:B5. LPS-B5 is a prototypical endotoxin and is often used as an endotoxin standard in Limulus amebocyte lysate (LAL) assays.
LPS is the principal component of Gram negative bacteria that activates the innate immune system. LPS recognition is predominantly mediated by TLR4 [1].

LPS-B5 is highly pyrogenic [2] and a potent activator of TLR4 with the subsequent induction of NF-κB and the production of pro-inflammatory cytokines [3, 4]. LPS-B5 is extracted by successive enzymatic hydrolysis steps and purified by the phenol-TEA-DOC extraction protocol as described previously [5]. LPS-B5 contains other bacterial components, such as lipoproteins, and therefore stimulates both TLR4 and TLR2.

InvivoGen provides LPS-B5 with two grades of purity:

  • LPS-B5 is a standard lipopolysaccharide (LPS) preparation. LPS-B5 contains other bacterial components, such as lipoproteins, and therefore stimulates both TLR4 and TLR2.
  • LPS-B5 Ultrapure underwent enzymatic treatment to remove lipopeptides and hence only activates TLR4.

 

References:

1. Poltorak A. et al., 1998. Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4 gene. Science, 282: 2085-8.
2. Dogan M. et al., 2000. Effects of different serotypes of Escherichia coli lipopolysaccharides on body temperature in rats. Life Sci. 67(19):2319-29.
3. Gutschow M. et al., 2013. Single-cell and population NF-κB
dynamic responses depend on lipopolysaccharide preparation. PLoS One. 8:e53222.
4. Hsu R. et al., 2011. LPS-induced TLR4 signaling in human colorectal cancer cells increases beta1 integrin-mediated cell adhesion and liver metastasis. Cancer Res. 1:1989- 98.
5. Hirschfeld M. et al., 2000. Cutting edge: repurification of lipopolysaccharide eliminates signaling through both human and murine toll-like receptor 2. J Immunol. 165(2):618-22..

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Specifications

Specificity:
LPS-B5 (Standard): TLR2/TLR4 agonist
LPS-B5 Ultrapure: TLR4 agonist

Working concentrations :
LPS-B5 (Standard):

• TLR4 activity: 100 pg - 1 μg/ml
• TLR2 activity: 100 ng/ml - 1mg/ml
LPS-B5 Ultrapure:
• TLR4 activity: 100 pg - 1 μg/ml

Quality control :
- The TLR4 activity is validated using HEK-Blue™ TLR4 cells.
- The presence of other bacterial components (e.g. lipoproteins) is monitored using HEK-Blue™ TLR2 cells.

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Contents

LPS-B5 (Standard):
- 5 mg LPS-B5 (lipopolysaccharide from E. coli serotype 055:K59(B5)H-)
Source strain: ATCC 12014; CDC 5624-50 [NCTC 9701]
- 1.5 ml endotoxin-free water

LPS-B5 Ultrapure:
- 5 mg LPS-B5 Ultrapure (Lipopolysaccharide from E. coli serotype 055:K59(B5)H-)
Source strain: ATCC 12014; CDC 5624-50 [NCTC 9701]
- 1.5 ml endotoxin-free water

room temperature LPS-B5 is shipped at room temperature

store Stored at -20 °C.

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