Class C CpG oligonucleotide - Stimulatory human / mouse TLR9 ligand
InvivoGen provides ODN 2395, a Class C CpG oligonucleotide (ODN) with a preference for the human and mouse Toll-like receptor 9 (TLR9). Class C CpG ODNs, such as ODN 2395, combine classes A and B features. They contain a complete phosphorothioate (PS) backbone and a CpG-containing palindromic motif.
CpG ODNs are synthetic oligonucleotides that contain unmethylated CpG dinucleotides in specific sequence contexts (CpG motifs). These CpG motifs are present at a 20-fold greater frequency in microbial DNA than in mammalian DNA. CpG ODNs are recognized by TLR9, which leads to strong immunostimulatory effects.
The sequence of the ODN 2395 is 5’-tcgtcgttttcggcgc:gcgccg-3’ of 22 mer. The bases are phosphorothioate (nuclease resistant). The palindrome is underlined.
Key features of InvivoGen's ODN 2395 products
- Potent activator of human and mouse TLR9
- Functional tests of each lot
- Negative control ODN available
- Provided in unmodified or FITC-labeled form
