Class A CpG oligonucleotide - Human TLR9 ligand
CpG ODNs are synthetic oligonucleotides that contain unmethylated CpG dinucleotides in specific sequence contexts (CpG motifs). These CpG motifs are present at a 20-fold greater frequency in bacterial DNA than in mammalian DNA. CpG ODNs are recognized by Toll-like receptor 9 (TLR9), which leads to strong immunostimulatory effects.
ODN 2216 is a class A CpG ODN and is a ligand of choice for human TLR9. Class A CpG ODNs are characterized by a central, phosphodiester, CpG-containing palindromic motif and a 3’ phosphorothioate poly-G string.They induce high IFN-α production from plasmacytoid dendritic cells (pDCs) but are weak stimulators of TLR9-dependent NF-κB signaling.