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ODN 2216 Biotin

ODN 2216 Biotin Unit size Cat. code Docs Qty Price
Class A - Biotinylated CpG ODN 2216
2 x 50 µg
tlrl-2216b
+-
$151.00

Biotin labeled CpG oligonucleotide - Human TLR9 ligand

CpG  ODNs are synthetic oligonucleotides that contain unmethylated CpG  dinucleotides in particular sequence contexts (CpG motifs).

These CpG  motifs are present at a 20-fold greater frequency in bacterial DNA  compared to mammalian DNA. They have been shown to induce a coordinated  set of immune responses based on the activation of immune cells  primarily involved in the recognition of these molecules.

ODN 2216 Biotin is a A-class CpG ODN with a preference for human TLR9.

ODN 2216 Biotin can be used to evaluate CpG ODN cellular uptake and localization using a biotin detection system and light microscopy.

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Specifications

Specificity: Human TLR9 agonist

Working concentration: 1-5 μM

ODN 2216 sequence : 5’-ggGGGACGA:TCGTCgggggg-3’ (20 mer)
Note: Bases shown in capital letters are phosphodiester, and those in lower case are phosphorothioate (nuclease resistant). Palindrome is underlined.

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Contents

  • 2 x 50 μg (7 nmol) lyophilized ODN 2216 labeled with Biotin at the 3’ terminus
  • 1 ml sterile endotoxin-free water

room temperature ODN 2216 Biotin is shipped at room temperature

store Stored at -20°C. Upon resuspension, prepare aliquots of ODN 2216 Biotin and store at -20°C.

stable Lyophilized ODN 2216 Biotin is stable for at least 1 year.  Resuspended product is stable 6 months when properly stored.

Alert Avoid repeated freeze-thaw cycles.

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