ODN 2216 Biotin
|ODN 2216 Biotin||Unit size||Cat. code||Docs||Qty||Price|
Class A - Biotinylated CpG ODN 2216
2 x 50 µg
Biotin labeled CpG oligonucleotide - Human TLR9 ligand
CpG ODNs are synthetic oligonucleotides that contain unmethylated CpG dinucleotides in particular sequence contexts (CpG motifs).
These CpG motifs are present at a 20-fold greater frequency in bacterial DNA compared to mammalian DNA. They have been shown to induce a coordinated set of immune responses based on the activation of immune cells primarily involved in the recognition of these molecules.
ODN 2216 Biotin is a A-class CpG ODN with a preference for human TLR9.
ODN 2216 Biotin can be used to evaluate CpG ODN cellular uptake and localization using a biotin detection system and light microscopy.
Specificity: Human TLR9 agonist
Working concentration: 1-5 μM
ODN 2216 sequence : 5’-ggGGGACGA:TCGTCgggggg-3’ (20 mer)
Note: Bases shown in capital letters are phosphodiester, and those in lower case are phosphorothioate (nuclease resistant). Palindrome is underlined.
- 2 x 50 μg (7 nmol) lyophilized ODN 2216 labeled with Biotin at the 3’ terminus
- 1 ml sterile endotoxin-free water
ODN 2216 Biotin is shipped at room temperature
Stored at -20°C. Upon resuspension, prepare aliquots of ODN 2216 Biotin and store at -20°C.
Lyophilized ODN 2216 Biotin is stable for at least 1 year. Resuspended product is stable 6 months when properly stored.
Avoid repeated freeze-thaw cycles.Back to the top