ODN 2243 (ODN 2216 Control)

Negative control for ODN 2216

ODN 2243 (also known as ODN 2216 Control) is designed as a negative control for the TLR9 agonist ODN 2216.

ODN 2216 is an A-class CpG ODN with a preference for human TLR9. A-class CpG ODNs are characterized by a phosphodiester central CpG-containing palindromic motif and a phosphorothioate 3’ poly-G string.They induce high IFN-α production from plasmacytoid dendritic cells (pDC) but are weak stimulators of TLR9-dependent NF-κB signaling [1-3].

ODN 2243 (ODN 2216 Control) contains GpC dinucleotides instead of CpGs and does not induce TLR9 activity [3-5].

References:

1. Krug A. et al., 2001. Identification of CpG oligonucleotide sequences with high induction of IFN-alpha/beta in plasmacytoid dendritic cells. Eur. J. Immunol. 31:2154-63.
2. Ballas Z. et al., 1996. Induction of NK activity in murine and human cells by CpG motifs in oligodeoxynucleotides and bacterial DNA. J. Immunol. 157:1840-5.
3. Vollmer J. et al., 2004. Characterization of three CpG oligodeoxynucleotide classes with distinct immunostimulatory activities. Eur. J. Immunol. 34: 251–62.
4.Rothenfusser S. et al., 2004. CpG-A and CpG-B oligonucleotides differentially enhance human peptide-specific primary and memory CD8+ T-cell responses in vitro. Blood 103:2162-9.
5.Ayash-Rashkovsky M. et al., 2005. Enhanced HIV-1 specific immune response by CpG ODN and HIV-1 immunogen-pulsed dendritic cells confers protection in the Trimera murine model of HIV-1 infection. FASEB J. 19:1152-4.

Specifications

Working concentration: 1-5 µM

Solubility:  5 mg/ml in water

ODN 2243 (ODN 2216 Control) sequence : 5’- ggGGGAGCATGCTGgggggg -3’ (20 mer)
Note: Bases shown in capital letters are phosphodiester, and those in lower case are phosphorothioate (nuclease resistant).

Quality control:

• The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.

Contents

• 1 mg (154.50 nmol) lyophilized ODN 2243 (ODN 2216 Control)
• 1.5 ml sterile endotoxin-free water

ODN 2243 (ODN 2216 Control) is shipped at room temperature.

Upon receipt, store at -20°C.