TLR-expressing NF-κB–SEAP and IRF–Lucia reporter cells
HEK-Dual™ TLR cells are human embryonic kidney 293 (HEK293)-derived cell lines designed to simultaneously monitor the NF-κB and IRF responses induced by Toll-like receptors (TLRs), known to activate these two pathways, namely TLR3, 4, 7, 8, 9. This dual reporter system offers added value compared to HEK-Blue™ TLR cells which only allow the monitoring of NF-κB activation.
These cell lines express an NF-κB-inducible SEAP (secreted embryonic alkaline phosphatase) and an IRF-inducible Lucia luciferase reporter gene. Both reporter proteins are readily measurable in the cell culture supernatant when using QUANTI-Blue™, a SEAP detection reagent, and QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia luciferase detection reagent.
HEK-Dual™ TLR cells provide a rapid, sensitive, and reliable method to screen and validate TLR agonists or antagonists.
Key features
- Improved NF-κB response to their specific ligands
- Monitoring of TLR-dependent NF-κB and IRF activation
- Stable expression of human TLR
Applications
- Defining the role of TLR-dependent IRF and NF-κB signaling pathways
- Screening for novel TLR agonists and inhibitors
InvivoGen's cell lines are extensively tested for viability, stability, biological activity, and absence of mycoplasma to ensure strong and reproducible results. Moreover, we provide detailed handling and experimental procedures for all cell lines, to minimize the need for optimization or troubleshooting by the end user.
