CL075

TLR7/8 Agonist - Thiazoquinoline compound

CL075 (3M002) is a thiazoloquinolone derivative that stimulates TLR8 in human peripheral blood mononuclear cells.

It activates NF-κB and triggers preferentially the production of TNF-α and IL-12 ^[1].

CL075 seems also to induce the secretion of IFN-α through TLR7 but to a lesser extend.

It induces the activation of NF-κB at 0.4 µM (0.1 µg/ml) in TLR8-transfected HEK293 cells, and ~ 10 times more CL075 is required to activate NF-κB in TLR7-transfected HEK293 cells.

Reference:

1. Gorden KB. et al., 2005. Synthetic TLR agonists reveal functional differences between human TLR7 and TLR8. J Immunol. 174(3):1259-68.

Specifications

Specificity: human TLR7/8 and mouse TLR7 agonist

Working Concentration:

• 0.1 - 5 μg/ml CL075 for human TLR8 and mouse TLR7
• 0.5 - 5 μg/ml CL075 for human TLR7

Endotoxin level:  EndoFit™ (<0.001 EU/µg)

Solubility: 1 mg/ml in water

Formula: C13H13N3S

Molecular weight: 243.33

Contents

CL075 is provided lyophilized and is available in two quantities:

Catalog code:tlrl-c75

• 500 µg: tlrl-c75
• 2 ml of sterile endotoxin-free water

Catalog code:tlrl-c75-5

• 5 mg: tlrl-c75-5
• 10 ml of sterile endotoxin-free water

CL075 is provided as a pale yellow lyophilized powder and shipped at room temperature.

Store at -20°C.

Lyophilized product is stable 1 year at -20°C when properly stored.

Resuspended CL075 should be aliquoted and stored at -20°C.

Resuspended product is stable 6 months at -20°C.

Avoid repeated freeze-thaw cycles.