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CL307

CL307 Unit size Cat. code Docs Qty Price
TLR7 Agonist - Base analog
500 µg
tlrl-c307
+-
$115.00

TLR7 Agonist - Base analog

CL307 (N1-glycinyl [4-((6-amino-2-(butylamino)-8-hydroxy-9H-purin-9-yl)methyl) benzoyl] spermine) was generated by covalently linking a spermine to the hydroxyadenine compound CL264.

CL307 is a very potent TLR7 agonist. Titration experiments have showed that CL307 induces robust NF-κB activation even at concentrations as low as 20 nM (10 ng/ml).

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Specifications

Specificity: human/mouse TLR7 agonist

Synonym: N1-glycinyl [4-((6-amino-2- (butylamino)-8- hydroxy-9H- purin- 9-yl)methyl) benzoyl] spermine

Working concentration: 5 ng- 1 µg/ml (~10nM - 2 µM)

Solubility:H2O (1 mg/ml)

Formula:C29H47N11O3

Molecular weight: 597 g/mol

Endotoxin level: <0.001 EU/µg

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Contents

  • 500 µg CL307
  • 1.5 ml sterile endotoxin-free water

room temperature Products are shipped at room temperature

store Stored at -20°C.

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Description

CL307 chemical structure

CL307 chemical structure

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Details

HEK-Blue™ hTLR7 cells stimulation

HEK-Blue™ hTLR7 cells, which stably express an NF-κB-inducible SEAP reporter gene and human TLR7 were incubated in HEK-Blue™ Detection (a SEAP detection growth medium) and stimulated with increasing concentrations of the agonists indicated in the graph.
After 24h incubation, the levels of NF-κB-induced SEAP were determined by reading the OD at 655 nm.

RAW-Blue™ cells, which stably express an NF-kB-inducible SEAP reporter gene, were stimulated with 0.6 mg/ml of multi-PRR ligands

RAW-Blue™ cells, which stably express an NF-κB-inducible SEAP reporter gene, were stimulated with 0.6 µg/ml of InvivoGen’s multi-PRR ligands complexed with 0.1 µg/ml HSV-60 (synthetic dsDNA).
After 24h incubation, the levels of NF-κB-induced SEAP were determined using QUANTI-Blue™, a SEAP detection reagent.

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