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ssPolyU / LyoVec™

TLR8 Agonist - RNA homopolymer / LyoVec™

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4 x 25 µg


TLR7 and/or TLR8 activation with ssRNA
TLR7 and/or TLR8 activation with ssRNA

TLR8 Agonist - RNA homopolymer - complexed with LyoVec™

ssPolyU/LyoVec™ is a lyophilized and complexed preparation of single-stranded poly-uridine (ssPolyU), able to activate the Toll-like receptors TLR7 and/or TLR8. 

Single-stranded RNA (ssRNA) has been identified as the natural ligand of TLR7 and TLR8 [1]. ssRNA derived from HIV-1 or the influenza virus were shown to induce the production of proinflammatory cytokines in pDC [2,3]. This induction was reproduced using polyU or GU-rich oligoribonucleotides (ORNs).



InvivoGen's ssPolyU is complexed with the cationic lipid LyoVec™ to protect it from degradation and facilitate its uptake. ssPolyU/LyoVec™ is a specific agonist of human TLR8 as verified by using our HEK-Blue™ reporter cell lines (see figure). Moreover, it activates mouse TLR7 [data not shown].

InvivoGen also offers a non-complexed version ssPolyU Naked.


Key features of ssPolyU/LyoVec™

  • Specific activator of human TLR8 
  • No activation of human TLR7
  • Non-complexed ssPolyU Naked is available
  • Complexed with LyoVec™ 
  • Each lot of ssPolyU/LyoVec™ is functionally tested


More infoRead our review about TLR7 and TLR8.



1. Heil F. et al., 2004. Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8. Science. 5;303(5663):1526-9.
2. Alter G. et al., 2007. Single-Stranded RNA Derived from HIV-1 Serves as a Potent Activator of NK Cells. J Immunol. 178:7658-7666.
3. Hornung V. et al., 2005. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7.Nat Med. 11(3):263-70.


NF-κB response of HEK-Blue™-derived cells to ssPolyU/LyoVec™
NF-κB response of HEK-Blue™-derived cells to ssPolyU/LyoVec™

NF-κB response of HEK-Blue™-derived cells to ssPolyU/LyoVec™. HEK-Blue™ cells expressing hTLR7 or hTLR8 were stimulated with increasing concentrations of ssPolyU/LyoVec™. After 24h incubation, the NF-κB-induced SEAP activity was assessed using QUANTI-Blue™. Data are shown as optical density (OD) at 650 nm (mean ± SEM). Of note, HEK-Blue™ Null* comprises data from parental cell lines HEK-Blue Null1 and HEK-Blue Null1-v.

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Specificity: human TLR8/mouse TLR7 agonist

Working Concentration: 1- 10 μg/ml

Solubility: 0.05 mg/ml in water

Quality control:

  • The biological activity of ssPolyU/LyoVec™ has been validated using cellular assays.
  • The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ hTLR2 and HEK-Blue™ hTLR4 cells.
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  • 4 x 25 μg lyophilized ssPolyU/LyoVec™

Note: Each vial contains 25 μg of ssPolyU complexed with 50 μg LyoVec™.

  • 10 ml endotoxin-free water Storage and stability:

room temperature ssPolyU/LyoVec™ is provided lyophilized and shipped at room temperature.

store Store lyophilized ssPolyU/LyoVec™ at -20 ̊C.

stability Lyophilized product is stable 1 year when stored -20 ̊C.

Upon resuspension, store ssPolyU/LyoVec™ at 4°C. Resuspended product is stable at 1 week at 4 °C.

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