ssPolyU / LyoVec™
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Cat.code:
tlrl-lpu
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ABOUT
TLR8 Agonist - RNA homopolymer - complexed with LyoVec™
ssPolyU/LyoVec™ is a lyophilized and complexed preparation of single-stranded poly-uridine (ssPolyU), able to activate the Toll-like receptors TLR7 and/or TLR8.
Single-stranded RNA (ssRNA) has been identified as the natural ligand of TLR7 and TLR8 [1]. ssRNA derived from HIV-1 or the influenza virus were shown to induce the production of proinflammatory cytokines in pDC [2,3]. This induction was reproduced using polyU or GU-rich oligoribonucleotides (ORNs).
Description
InvivoGen's ssPolyU is complexed with the cationic lipid LyoVec™ to protect it from degradation and facilitate its uptake. ssPolyU/LyoVec™ is a specific agonist of human TLR8 as verified by using our HEK-Blue™ reporter cell lines (see figure). Moreover, it activates mouse TLR7 [data not shown].
InvivoGen also offers a non-complexed version ssPolyU Naked.
Key features of ssPolyU/LyoVec™
- Specific activator of human TLR8
- No activation of human TLR7
- Non-complexed ssPolyU Naked is available
- Complexed with LyoVec™
- Each lot of ssPolyU/LyoVec™ is functionally tested
Read our review about TLR7 and TLR8.
References:
1. Heil F. et al., 2004. Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8. Science. 5;303(5663):1526-9.
2. Alter G. et al., 2007. Single-Stranded RNA Derived from HIV-1 Serves as a Potent Activator of NK Cells. J Immunol. 178:7658-7666.
3. Hornung V. et al., 2005. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7.Nat Med. 11(3):263-70.
All products are for research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
1-10 μg/ml
0.05 mg/ml in water
cellular assays
Each lot is functionally tested and validated.
CONTENTS
Contents
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Product:ssPolyU / LyoVec™
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Cat code:tlrl-lpu
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Quantity:4 x 25 µg
10 ml endotoxin-free water.
Each vial contains 25 μg of ssPolyU complexed with 50 μg LyoVec™.
Shipping & Storage
- Shipping method: Room temperature
- Upon receipt, store at -20°C.
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
DOCUMENTS
Documents
Technical Data Sheet
Safety Data Sheet
Validation Data Sheet
Certificate of analysis
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