ssPolyU/LyoVec™
| ssPolyU / LyoVec™ | Unit size | Cat. code | Docs | Qty | Price |
|---|---|---|---|---|---|
TLR8 Agonist - RNA homopolymer / LyoVec™ |
4 x 25 µg |
tlrl-lpu |
TLR8 Agonist - RNA homopolymer / LyoVec™
ssPolyU is a lyophilized preparation of single-stranded poly-uridine (polyU) complexed with the cationic lipid LyoVec™, to protect it from degradation and facilitate its uptake.
PolyU is a synthetic ssRNA which when complexed to cationic lipids can substitute for viral RNAs in inducing IFN-α production in plasmacytoid dendritic cells (pDC).
Murine PDC deficient for TLR7 failed to produce IFN-α in response to polyU, while the response to CpG-ODNs was unaffected, suggesting that TLR7 plays a critical role in viral ssRNA recognition [1].
In human cells, TLR8 was shown to be the key receptor for viral ssRNA [2], implying a species specificity difference in ssRNA recognition.
During infection, some viral particles are degraded by the endosomal proteases, exposing the viral genome and allowing TLR7 and/or TLR8 signaling, which are known to occur in endosomes [3].
TLR7 and TLR8 can recognize both self and viral RNA but seem able to distinguish the presence of viral RNA by detecting their abnormal localization in the endosome rather than a particular RNA motif.
References:
1. Diebold SS. et al., 2004. Innate antiviral responses by means of TLR7-mediated recognition of single-stranded RNA. Science. 5;303(5663):1529-31
2. Heil F. et al., 2004. Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8. Science. 5;303(5663):1526-9.
3. Heil F. et al., 2003 The Toll-like receptor 7 (TLR7)-specific stimulus loxoribine uncovers a strong relationship within the TLR7, 8 and 9 subfamily. Eur J Immunol. 33(11):2987-97.
Specifications
Specificity: human TLR8/mouse TLR7 agonist
Working Concentration: 1- 10 μg/ml
Solubility: 0.05 mg/ml in water
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- 4 x 25 μg lyophilized ssPolyU/LyoVec™
Note: Each vial contains 25 μg of ssPolyU complexed with 50 μg LyoVec™.
- 10 ml endotoxin-free water Storage and stability:
ssPolyU/LyoVec™ is provided lyophilized and shipped at room temperature.
Store lyophilized ssPolyU/LyoVec™ at -20 ̊C.
Lyophilized product is stable 1 year when stored -20 ̊C.
Upon resuspension, store ssPolyU/LyoVec™ at 4°C. Resuspended product is stable at 1 week at 4 °C.
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