ssPolyU/LyoVec™ - TLR8 Agonist

RNA homopolymer / LyoVec™ complexed

ABOUT

TLR8 Agonist - RNA homopolymer - complexed with LyoVec™

ssPolyU/LyoVec™ is a lyophilized and complexed preparation of single-stranded poly-uridine (ssPolyU), able to activate the Toll-like receptors TLR7 and/or TLR8.

Single-stranded RNA (ssRNA) has been identified as the natural ligand of TLR7 and TLR8 [1]. ssRNA derived from HIV-1 or the influenza virus were shown to induce the production of proinflammatory cytokines in pDC [2,3]. This induction was reproduced using polyU or GU-rich oligoribonucleotides (ORNs).

 

Description

InvivoGen's ssPolyU is complexed with the cationic lipid LyoVec™ to protect it from degradation and facilitate its uptake. ssPolyU/LyoVec™ is a specific agonist of human TLR8 as verified by using our HEK-Blue™ reporter cell lines (see figure). Moreover, it activates mouse TLR7 [data not shown].

InvivoGen also offers a non-complexed version ssPolyU Naked.

 

Key features of ssPolyU/LyoVec™

  • Specific activator of human TLR8
  • No activation of human TLR7
  • Non-complexed ssPolyU Naked is available
  • Complexed with LyoVec™
  • Each lot of ssPolyU/LyoVec™ is functionally tested

 

More info Read our review about TLR7 and TLR8.

 

References:

1. Heil F. et al., 2004. Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8. Science. 5;303(5663):1526-9.
2. Alter G. et al., 2007. Single-Stranded RNA Derived from HIV-1 Serves as a Potent Activator of NK Cells. J Immunol. 178:7658-7666.
3. Hornung V. et al., 2005. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7.Nat Med. 11(3):263-70.

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Source
Single-stranded poly-uridine (ssPolyU) complexed with LyoVec™
Working concentration

1-10 μg/ml

Solubility

0.05 mg/ml in water

Tested applications

cellular assays

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    ssPolyU / LyoVec™
  • Cat code: 
    tlrl-lpu
  • Quantity: 
    4 x 25 µg
Includes:

10 ml endotoxin-free water.

Notes:

Each vial contains 25 μg of ssPolyU complexed with 50 μg LyoVec™.

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • Upon receipt, store at -20°C.
    Stability: Lyophilized product is stable 1 year when stored -20 ̊C. Upon resuspension, store ssPolyU/LyoVec™ at 4°C. Resuspended product is stable at 1 week at 4 °C.

    Caution:

    • Avoid repeated freeze-thaw cycles

DOCUMENTS

Documents

ssPolyU / LyoVec™

Technical Data Sheet

Safety Data Sheet

Validation Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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