ssPolyU Naked
| ssPolyU Naked | Unit size | Cat. code | Docs | Qty | Price |
|---|---|---|---|---|---|
TLR8 Agonist - RNA homopolymer |
10 mg |
tlrl-sspu |
TLR8 Agonist - RNA homopolymer
Poly(U) is a synthetic ssRNA which can substitute for viral RNAs in inducing IFN-α production in plasmacytoid dendritic cells.
Stimulation of mouse TLR7 and human TLR8 can be achieved with poly(U) in cells expressing human TLR8 or cell expressing mouse TLR7.
To achieve poly(U) stimulation of hTLR8 or mTLR7, the poly(U) must be delivered intracellularly, for exemple example by using a transfection agent, such as LyoVec™.
Back to the topSpecifications
Specificity: human TLR8/mouse TLR7 agonist
Working Concentration: 1 - 10 μg/ml
CAS number: 28086-43-3
Solubility: 1 mg/ml in water
Back to the topContents
- 10 mg of lyophilized Poly(U)
- 10 ml of endotoxin-free water
Poly(U) is shipped at room temperature.
Store lyophilized Poly(U) at -20 °C.
Lyophilized product is stable 1 year when stored -20 ̊C.
Description
Poly(U) is a synthetic ssRNA which can substitute for viral RNAs in inducing IFN-α production in plasmacytoid dendritic cells (pDC). Murine pDC deficient for TLR7 failed to produce IFN-α in response to poly(U), while the response to CpG-ODNs was unaffected, suggesting that TLR7 plays a critical role in viral ssRNA recognition [1].
In human cells, TLR8 was shown to be the key receptor for viral ssRNA [2], implying a species specificity difference in ssRNA recognition. During infection, some viral particles are degraded by the endosomal proteases, exposing the viral genome and allowing TLR7 and/or TLR8 signaling, which are known to occur in endosomes [3].
TLR7 and TLR8 can recognize both self and viral RNA but seem able to distinguish the presence of viral RNA by detecting their abnormal localization in the endosome rather than a particular RNA motif.
1. Diebold SS. et al., 2004. Innate antiviral responses by means of TLR7-mediated recognition of single-stranded RNA. Science. 5;303(5663):1529-31
2. Heil F. et al., 2004. Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8. Science. 5;303(5663):1526-9.
3. Heil F. et al., 2003. The Toll-like receptor 7 (TLR7)- specific stimulus loxoribine uncovers a strong relationship within the TLR7, 8 and 9 subfamily. Eur J Immunol. 33(11):2987-97.