ssPolyU Naked - TLR8 Agonist

TLR8 Agonist - RNA homopolymer - non-complexed

Poly(U) is a lyophilized preparation of a synthetic single-stranded poly-uridine (ssPolyU), able to activate the Toll-like receptors TLR7 and/or TLR8.

Single-stranded RNA (ssRNA) has been identified as the natural ligand of TLR7 and TLR8 [1]. ssRNA derived from HIV-1 or the influenza virus were shown to induce the production of proinflammatory cytokines in pDC [2,3]. This induction was reproduced using polyU or GU-rich oligoribonucleotides (ORNs).

Description

InvivoGen's ssPolyU Naked is not complexed and therefore, not protected from degradation. Still, ssPolyU Naked is a specific agonist of human TLR8 as verified by using our HEK-Blue™ reporter cell lines (see figure). Moreover, it activates mouse TLR7 [data not shown].

InvivoGen also offers a complexed version ssPolyU/LyoVec™

Key features of ssPolyU Naked

• Specific activator of human TLR8
• No activation of human TLR7
• Complexed ssPolyU/LyoVec™ is available
• Non-complexed
• Each lot of ssPolyU Naked is functionally tested

Read our review about TLR7 and TLR8.

References:

1. Heil F. et al., 2004. Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8. Science. 5;303(5663):1526-9.
2. Alter G. et al., 2007. Single-Stranded RNA Derived from HIV-1 Serves as a Potent Activator of NK Cells. J Immunol. 178:7658-7666.
3. Hornung V. et al., 2005. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7.Nat Med. 11(3):263-70.