ORN 06 / LyoVec™
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Cat.code:
tlrl-orn6
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ABOUT
TLR7/8 Agonist - minimal ssRNA motif of ssRNA40 with 6 UUGU repeats - complexed with LyoVec™
The oligoribonucleotide ORN06 contains six repeats of the UUGU sequence motif, identified as the minimal motif responsible for ssRNA40 immunoreactivity. ssRNA40, also known as R-1075, is a U-rich single-stranded (ss)RNA derived from the long terminal repeat of HIV-1, and a strong agonist of the human (h) Toll-like receptor 8 (TLR8) [1].
ssRNA derived from HIV-1 or the influenza virus were shown to induce the production of proinflammatory cytokines by activation of TLR7 and/or TLR8 [2].
More details
Description
InvivoGen's ORN06 is complexed with the cationic lipid LyoVec™ to protect it from degradation and facilitate its uptake. Moreover, phosphorothioate linkages were incorporated in order to extend the effective molecular lifetime by minimizing extra and intracellular nuclease degradation. ORN06/LyoVec™ is a strong agonist of human TLR8 and mouse TLR7, as verified by using our HEK-Blue™ reporter cell lines. Moreover, it slightly activates human TLR7 in our HEK-derived cells (see figure).
Key features of ORN06/LyoVec™
- Strong activator of human TLR8 and mouse TLR7
- Complexed with LyoVec™
- Each lot of ORN06/LyoVec™ is functionally tested
Read our review about TLR7 and TLR8.
References:
1. Forsbach A, et al., 2008. Identification of RNA sequence motifs stimulating sequence-specific TLR8-dependent immune responses. J Immunol. 2008 Mar 15;180(6):3729-38.
2. Heil F. et al., 2004. Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8. Science. 5;303(5663):1526-9.
All products are for internal research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
TLR7, TLR8
Human, Mouse
5’-U*U*G*U*U*G*U*U*G*U*U*G*U*U*G*U*U*G*U*U-3’ (20 mer)
* depicts the phosphorothioate linkage
0.25 - 5 μg/ml
0.05 mg/ml in water
Negative (tested using EndotoxDetect™ assay)
Cellular assays
TLR7 activation
TLR8 activation
Each lot is functionally tested and validated.
complexed with transfection reagent LyoVec™
CONTENTS
Contents
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Product:ORN 06 / LyoVec™
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Cat code:tlrl-orn6
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Quantity:4 x 25 µg
10 ml endotoxin-free water
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
Details
Single-stranded RNA (ssRNA) has been identified as the natural ligand of TLR7 and TLR8 [1, 2]. ssRNA derived from HIV-1 or the influenza virus were shown to induce the production of proinflammatory cytokines in pDC.
This induction was reproduced using U-rich single-stranded RNAs, such as polyU or GU-rich (ssRNA40) ODNs complexed with cationic lipids to protect them from degradation.
According to Diebold et al., this activation is sequence-independent as long as the ssRNAs contain several uridines in close proximity [1].
However, recent studies suggest sequence-dependent recognition of U-rich ssRNAs by TLR7 and TLR8.
They demonstrate that AU-rich and GU-rich oligoribonucleotides (ORNs) are capable of activating TLR8, whereas only GU-rich ORNs are able to stimulate TLR7 [3].
ORN 06 contains 6 repeats of the UUGU sequence motif, identified as the minimal motif responsible for ssRNA40 immunoactivity [3].
1. Diebold S. et al., 2006. Nucleic acid agonists for Toll-like receptor 7 are defined by the presence of uridine ribonucleotides. Eur. J. Immunol., 36:3256-67.
2. Heil F. et al., 2004. Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8. Science. 5;303(5663):1526-9.
3. Forsbach A. et al., 2008. Identification of RNA Sequence Motifs Stimulating Sequence-Specific TLR8-Dependent Immune Responses. J. Immunol., 180: 3729-38.
DOCUMENTS
Documents
Technical Data Sheet
Validation Data Sheet
Safety Data Sheet
Certificate of analysis
Need a CoA ?
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