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ORN06/LyoVec™

ORN 06 / LyoVec™ Unit size Cat. code Docs Qty Price
TLR8 Agonist - ssRNA with 6 UUGU repeats / LyoVec™
4 x 25 µg
tlrl-orn6
+-
$306.00

TLR8 Agonist - ssRNA with 6 UUGU repeats / LyoVec™

ORN 06 contains 6 repeats of the UUGU sequence motif, identified as the minimal motif responsible for ssRNA40 immunoactivity.

ORN06 is complexed with the cationic lipid LyoVec™, to protect it from degradation and facilitate it uptake.

ORN 06/LyoVec™ has been tested using HEK-Blue™ TLR7 and HEK-Blue™ TLR8 Cells to confirm its specificity.

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Specifications

Specificity: human TLR8/mouse TLR7 agonist

Working Concentration: 0.25 - 5 μg/ml

Sequence: ORN 06 5’-UUGUUGUUGUUGUUGUUGUU-3’ (20 mer)

Solubility: 0.05 mg/ml in water

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Contents

  • 4 x 25 mg lyophilized ORN 06/LyoVec™
  • 10 ml endotoxin-free water

room temperature ORN 06/LyoVec™ is provided lyophilized and shipped at room temperature.

store Store at -20°C.

stability Lyophilized product is stable 1 year at -20 ̊C.

Upon resuspension, store product at 4°C. Resuspended product is stable 1 week at 4°C.

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Description

Single-stranded RNA (ssRNA) has been identified as the natural ligand of TLR7 and TLR8 [1, 2]. ssRNA derived from HIV-1 or the influenza virus were shown to induce the production of proinflammatory cytokines in pDC.

This induction was reproduced using U-rich single-stranded RNAs, such as polyU or GU-rich (ssRNA40) ODNs complexed with cationic lipids to protect them from degradation.

According to Diebold et al., this activation is sequence-independent as long as the ssRNAs contain several uridines in close proximity [1].

However, recent studies suggest sequence-dependent recognition of U-rich ssRNAs by TLR7 and TLR8.

They demonstrate that AU-rich and GU-rich oligoribonucleotides (ORNs) are capable of activating TLR8, whereas only GU-rich ORNs are able to stimulate TLR7 [3].

ORN 06 contains 6 repeats of the UUGU sequence motif, identified as the minimal motif responsible for ssRNA40 immunoactivity [3].

 

1. Diebold S. et al., 2006. Nucleic acid agonists for Toll-like receptor 7 are defined by the presence of uridine ribonucleotides. Eur. J. Immunol., 36:3256-67.
2. Heil F. et al., 2004. Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8. Science. 5;303(5663):1526-9.
3. Forsbach A. et al., 2008. Identification of RNA Sequence Motifs Stimulating Sequence-Specific TLR8-Dependent Immune Responses. J. Immunol., 180: 3729-38.

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Citations

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