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LyoVec™

LyoVec™ Unit size Cat. code Docs Qty Price
Cationic lipid for transfection of mammalian cells + 1 vial of control LyoVec™-GFP/SEAP complex
8ml, 160 reactions in 12-well plates
10 ml, 200 reactions in 12-well plates
18 ml, 360 reactions in 12-well plates
20 ml, 400 reactions in 12-well plates
16 ml, 320 reactions in 12-well plates
lyec-12
+-
$128.00

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How to use LyoVec™
How to use LyoVec™

Lyophilized Lipid-based Transfection Reagent

LyoVec™ is a proprietary cationic lipid-based transfection reagent belonging to the family of phosphonolipids. This family was originally described by Floch et al., as efficient transfection reagents for both in vitro and in vivo use [1-2].

The major constituent of LyoVec™ is the phosphonolipid DTCPTA, which is coupled to DiPPE, a neutral lipid that helps destabilize membrane bilayers and increases the in vitro transfection efficiency of LyoVec™.

Applications of LyoVec™:

  • Cationic lipid-based transfection reagent
  • Nucleic acid complexing agent

The positive charge of the phosphonolipid enables LyoVec™ to bind to plasmid DNA, and the structure promotes fusion with cellular membranes for efficient DNA delivery.

Additionally, LyoVec™ can be used as a nucleic acid complexing agent to facilitate the cellular entry of RNA or DNA-based oligonucleotides, such as RIG-I ligands (e.g. poly(dA:dT) [3] and poly(I:C) [4]) and endosomal TLR7/8 ligands (e.g. ssPolyU [5]). This complexation step is crucial for inducing an effective response to the nucleic acids by pathogen recognition receptors.

 

References

1. Floch et al., 1997. Cationic phosphonolipids as non-viral vectors for DNA transfection in hematopoietic cell lines and CD34+ cells. Blood Cells Molec.& Diseases 23:69-87.
2. Guillaume-Gable et al., 1998. Cationic phosphonolipids as nonviral gene transfer agents in the lung of mice. Hum Gene Ther 9:2309-2319.
3. Xie M. et al., 2016. PKM2-dependent  glycolysis  promotes  NLRP3  and  AIM2  inflammasome  activation.  Nat Commun. 7:13280. 
4. Järver P. et al., 2018. Single-Stranded Nucleic Acids Regulate TLR3/4/7  Activation  through  Interference  with  Clathrin-Mediated  Endocytosis. Sci  Rep.  8:15841.
5.  Katashiba et al., 2011. Interferon-α and interleukin-12 are induced,  respectively,  by  double-stranded DNA  and  single-stranded RNA  in human myeloid dendritic cells. Immunology. 132(2):165-73.

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Specifications

Hands-on time: No more than 15 min and no preparation required the day prior transfection

Transfection: Transient and stable transfections

Conditions: Works in the presence of serum, with no need to wash cells after transfection

Shelf life: Long shelf-life of plasmid DNA (pDNA)/ LyoVec™ complexes

Quality Control:

  • Transfection efficiency of LyoVec™ has been confirmed in HEK293‑derived cells. 
  • Cytotoxicity has been assessed in HEK293‑derived cells.
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Contents

LyoVec™ is provided as a lyophilized powder, sterile and packaged in sealed glass vials.

This product is available in different formats:

  • lyec-1 : 5 small vials (sufficient to perform 200 reactions)
  • lyec-2 : 10 small vials (sufficient to perform 400 reactions)
  • lyec-3 : 2 large vials (sufficient to perform 320 reactions)
  • lyec-12 : 4 small vials (sufficient to perform 160 reactions) and 1 vial of control LyoVec™-GFP/SEAP complex.
  • lyec-22 : 9 small vials (sufficient to perform 360 reactions) and 1 vial of control LyoVec™-GFP/SEAP complex.

 

LyoVec™ is shipped at room temperature.

Upon receipt, it should be stored at 4°C.

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Citations

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