LyoVec™

Lyophilized Lipid-based Transfection Reagent

LyoVec™ is a proprietary cationic lipid-based transfection reagent belonging to the family of phosphonolipids. This family was originally described by Floch et al., as efficient transfection reagents for both in vitro and in vivo use [1-2].

The major constituent of LyoVec™ is the phosphonolipid DTCPTA, which is coupled to DiPPE, a neutral lipid that helps destabilize membrane bilayers and increases the in vitro transfection efficiency of LyoVec™.

Applications of LyoVec™:

• Cationic lipid-based transfection reagent
• Nucleic acid complexing agent

The positive charge of the phosphonolipid enables LyoVec™ to bind to plasmid DNA, and the structure promotes fusion with cellular membranes for efficient DNA delivery.

Additionally, LyoVec™ can be used as a nucleic acid complexing agent to facilitate the cellular entry of RNA or DNA-based oligonucleotides, such as RIG-I ligands (e.g. poly(dA:dT) [3] and poly(I:C) [4]) and endosomal TLR7/8 ligands (e.g. ssPolyU [5]). This complexation step is crucial for inducing an effective response to the nucleic acids by pathogen recognition receptors.

References

1. Floch et al., 1997. Cationic phosphonolipids as non-viral vectors for DNA transfection in hematopoietic cell lines and CD34+ cells. Blood Cells Molec.& Diseases 23:69-87.
2. Guillaume-Gable et al., 1998. Cationic phosphonolipids as nonviral gene transfer agents in the lung of mice. Hum Gene Ther 9:2309-2319.
3. Xie M. et al., 2016. PKM2-dependent  glycolysis  promotes  NLRP3  and  AIM2  inflammasome  activation.  Nat Commun. 7:13280. 
4. Järver P. et al., 2018. Single-Stranded Nucleic Acids Regulate TLR3/4/7  Activation  through  Interference  with  Clathrin-Mediated  Endocytosis. Sci  Rep.  8:15841.
5.  Katashiba et al., 2011. Interferon-α and interleukin-12 are induced,  respectively,  by  double-stranded DNA  and  single-stranded RNA  in human myeloid dendritic cells. Immunology. 132(2):165-73.