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293XL/TLR7

293XL/hTLR7 Unit size Cat. code Docs Price
293XL cell line expressing the human TLR7 gene
3-7 x 10e6 cells
293xl-htlr7
293XL/mTLR7 Unit size Cat. code Docs Price
293XL cell line expressing the murine TLR7 gene
3-7 x 10e6 cells
293xl-mtlr7

HEK293 clones expressing TLR7

293XL/TLR7 cells were obtained by stable transfection of HEK293XL cells with a pUNO-TLR7 plasmid which expresses the human or murine TLR7 gene.

The control cell line of 293XL/TLR7 cells is 293XL/null cells.

Figures

TLR and NOD induction profile of 293 clones
TLR and NOD induction profile of 293 clones

293/TLR, NOD and control clones were transfected transiently with pNiFty-SEAP and stimulated with TLR and NOD ligands. After 16 hour stimulation, NF-κB-induced SEAP activity was assessed using QUANTI-Blue™, a SEAP detection medium.

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Specifications

Antibiotic resistance: blasticidin

Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™

Guaranteed mycoplasma-free

These products are covered by a Limited Use License (See Terms and Conditions).

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Contents

dry ice Shipped on dry ice (Europe, USA, Canada and some areas in Asia)

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Description

TLR7, which is abundantly expressed in lung, placenta, spleen and PBL, is phylogenetically close to TLR8 and TLR9 [1]. TLR7 recognizes small synthetic molecules such as loxoribine and R848, an imidazoquinoline compound [2]. TLR7 signaling involves the MyD88-dependent signaling cascade and induces the production of IFN-α, TNF-α and IL-12. Recently, single-stranded RNA (ssRNA) was identified as the natural ligand of TLR7 [3, 4]. ssRNA derived from HIV-1 or the influenza virus were shown to induce the production of proinflammatory cytokines in PDC. TLR7 signaling is abrogated by chloroquine indicating that it is dependent on endosomal acidification.

 

1. Chuang TH. and RJ. Ulevitch, 2000. Cloning and characterization of a sub-family of human toll-like receptors: hTLR7, hTLR8 and hTLR9. Eur Cytokine Netw, 11(3):372-8
2. Hemmi H. et al., 2002. Small anti-viral compounds activate immune cells via the TLR7 MyD88-dependent signaling pathway. Nat Immunol, 3(2):196-200
3. Heil F. et al., 2004. Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8. Science. 303(5663):1526-9.
4. Diebold SS. et al., 2004. Innate antiviral responses by means of TLR7-mediated recognition of single-stranded RNA. Science. 303(5663):1529-31.

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