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293/TLR9

293XL/hTLR9 Unit size Cat. code Docs Qty Price
293XL cell line expressing the human TLR9a gene
3-7 x 10e6 cells
293xl-htlr9
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$738.00
293/mTLR9 Unit size Cat. code Docs Qty Price
293 cell line expressing the murine TLR9 gene
3-7 x 10e6 cells
293-mtlr9
+-
$738.00

293/mTLR9 cells were obtained by stable transfection of HEK293 cells with a pUNO-TLR9 plasmid which expresses the murine TLR9 gene.
293XL/TLR9A cells were obtained by stable transfection of HEK293XL cells with a pUNO-TLR9 plasmid which expresses the human TLR9 gene.

The control cell line of 293/mTLR9 cells is 293/null.
The control cell line of 293XL/hTLR9A cells is 293XL/null cells.

 


293/TLR, NOD and control clones were transfected transiently with pNiFty-SEAP and stimulated with TLR and NOD ligands. After 16 hour stimulation, NF-κB-induced SEAP activity was assessed using QUANTI-Blue™, a SEAP detection medium.

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Specifications

Antibiotic resistance: blasticidin

Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™

Guaranteed mycoplasma-free

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Contents

Shipped on dry ice

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Description

TLR9 is expressed in the endoplasmic reticulum of resting cells in contrast to most TLRs that are located on the plasma membrane [1]. TLR9 recognizes specific unmethylated CpG-ODN sequences, that distinguish bacterial DNA from mammalian DNA. TLR9 engages an intracellular signaling pathway that involves MyD88 leading to NF-κB translocation [2]. TLR9 recognizes different CpG motifs; the optimal sequences being GTCGTT and GACGTT for human TLR9 and murine TLR9 respectively [3]. TLR9 binds directly to CpG DNA and recognizes specifically CpG DNA that is unmethylated and single stranded [4].

 

1. Latz E. et al., 2004. TLR9 signals after translocating from the ER to CpG DNA in the lysosome. Nat Immunol. 5(2):190-8.
2. Chuang TH. et al., 2002. Toll-like receptor 9 mediates CpG-DNA signaling. J Leukoc Biol, 71(3):538-44.
3. Bauer S. et al., 2001. Human TLR9 confers responsiveness to bacterial DNA via species-specific CpG motif recognition. Proc Natl Acad Sci USA, 98(16):9237-42.
4. Rutz M. et al., 2004. Toll-like receptor 9 binds single-stranded CpGDNA in a sequence- and pH-dependent manner. Eur J Immunol. 34(9):2541-50.

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Citations

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