Invivogen
Menu

293/TLR5

293/hTLR5 Unit size Cat. code Docs Qty Price
HEK 293 cells stably transfected with the human TLR5 gene
3-7 x 10e6 cells
293-htlr5
+-
$738.00
293/mTLR5 Unit size Cat. code Docs Qty Price
HEK 293 cells stably transfected with the murine TLR5 gene
3-7 x 10e6 cells
293-mtlr5
+-
$738.00

293/TLR5 cells were obtained by stable transfection of HEK293 cells with a pUNO-TLR5 plasmid which expresses the human or murine TLR5 gene.

The control cell line of 293/TLR5 cells is 293/null.


293/TLR, NOD and control clones were transfected transiently with pNiFty-SEAP and stimulated with TLR and NOD ligands. After 16 hour stimulation, NF-κB-induced SEAP activity was assessed using QUANTI-Blue™, a SEAP detection medium.

Back to the top

Specifications

Antibiotic resistance: blasticidin

Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™

Guaranteed mycoplasma-free

Back to the top

Contents

Shipped on dry ice

Back to the top

Description

TLR5 recognizes flagellin from both Gram-positive and Gram-negative bacteria. Activation of the receptor stimulates the production of proinflammatory cytokines, such as TNF-α, through signaling via the adaptor protein MyD88 and the serine kinase IRAK [1, 2]. TLR5 can generate a proinflammatory signal as a homodimer suggesting that it might be the only TLR participating in flagellin recognition [2]. However, TLR5 may require the presence of a co-receptor or adaptor molecule for efficient ligand recognition and/or signaling [3].

 

1. Gewirtz AT. et al., 2001. Cutting edge: bacterial flagellin activates basolaterally expressed TLR5 to induce epithelial proinflammatory gene expression. J Immunol, 167(4):1882-5
2. Hayashi F. et al., 2001. The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5. Nature, 410(6832):1099-103
3. Tallant T. et al., 2004. Flagellin acting via TLR5 is the major activator of key signaling pathways leading to NF-kappa B and proinflammatory gene program activation in intestinal epithelial cells. BMC Microbiol. 4(1):33.

Back to the top

Citations

Customer Service
& Technical Support

CAPTCHA
In order to prevent automated spam submissions, please answer the following question:
5 + 2 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.
Shopping cart is empty