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293/TLR2

293/hTLR2 Unit size Cat. code Docs Qty Price
HEK 293 cells stably transfected with the human TLR2 gene
3-7 x 10e6 cells
293-htlr2
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$738.00
293/mTLR2 Unit size Cat. code Docs Qty Price
HEK 293 cells stably transfected with the murine TLR2 gene
3-7 x 10e6 cells
293-mtlr2
+-
$738.00

293/TLR2 cells were obtained by stable transfection of HEK293 cells with a pUNO-TLR2 plasmid which expresses the human or murine TLR2 gene.

The control cell line for 293/hTLR2 and 293/mTLR2 cells is 293/null.


293/TLR, NOD and control clones were transfected transiently with pNiFty-SEAP and stimulated with TLR and NOD ligands. After 16 hour stimulation, NF-κB-induced SEAP activity was assessed using QUANTI-Blue™, a SEAP detection medium.

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Specifications

Antibiotic resistance: blasticidin

Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™

Guaranteed mycoplasma-free

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Contents

Shipped on dry ice

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Details

TLR and NOD induction profile in 293 clones

TLR and NOD induction profile of 293 clones :
293/TLR, NOD and control clones were transfected transiently with pNiFty-SEAP and stimulated with TLR and NOD ligands. After 16 hour stimulation, NF-κB-induced SEAP activity was assessed using QUANTI-Blue™, aSEAP-detection medium.

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Citations

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