293-Dual™ hSTING-A162 Cells
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Cat.code:
293d-a162
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ABOUT
293-Dual™ hSTING-A162 Cells (ISG-SEAP/KI-[IFN-β]Lucia)
293-Dual™ hSTING-A162 (ISG/KI-IFNb) cells were generated from 293-Dual™ Null (ISG/KI-IFNb) cells by stable transfection of the A162 isoform of human STING (S162A).
The allele A162 contains a unique point mutation (S162A) placed at the cyclic-dinucleotide-binding site which confers sensitivity to DMXAA, a potent tumor vascular disrupting agent in mice [2].
In the absence of this mutation, DMXAA has no effect on human STING [3].
293-Dual™ hSTING-A162 (ISG/KI-IFNb) cells are resistant to blasticidin, hygromycin, and Zeocin®.
References:
1. Yi G. et al., 2013. Single nucleotide polymorphisms of human STING can affect innate immune response to cyclic dinucleotides. PLoS One. 8(10):e77846.
2. Gao P. et al., 2013. Structure-function analysis of STING activation by c[G(2’,5’)pA(3’,5’)p] and targeting by antiviral DMXAA. Cell 154(4):748-62.
3. Conlon J. et al., 2013. Mouse, but not human STING, binds and signals in response to the vascular disrupting agent 5,6-dimethylxanthenone-4-acetic acid. J Immunol 190(10):5216-25.
Disclaimer: These cells are for internal research use only and are covered by a Limited Use License (See Terms and Conditions). Additional rights may be available.
SPECIFICATIONS
Specifications
STING activation cellular assays
Complete DMEM (see TDS)
Validated using PlasmotestTM
Each lot is functionally tested and validated.
CONTENTS
Contents
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Product:293-Dual™ hSTING-A162 Cells
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Cat code:293d-a162
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Quantity:3-7 x 10^6 cells
- 1 ml of Blasticidin (10 mg/ml)
- 1 ml of Hygromycin (100 mg/ml)
- 1 ml of Zeocin® (100 mg/ml)
- 1 ml of Normocin™ (50 mg/ml)
- 1 ml of QB reagent and 1 ml of QB buffer
- 1 tube of QUANTI-Luc™ 4 Reagent
Shipping & Storage
- Shipping method: Dry ice
- Liquid nitrogen vapor
Storage:
DOCUMENTS
Documents
Technical Data Sheet
Validation Data Sheet
Safety Data Sheet
Certificate of analysis
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