Lucia luciferase Reporter Cell Lines
InvivoGen has developed new reporter cell lines that feature the Lucia luciferase reporter gene.
These cell lines allow the study of one or two signal transduction pathways following their activation by diverse molecules such as microbial ligands or cytokines.
They were obtained by stable tranfection of the extensively used human embryonic kidney (HEK) 293 cell line or well-established immune cell models, such as the human monocytic THP-1 cell line.
Some of the cell lines express a dual reporter system containing the Lucia (secreted luciferase) and SEAP (secreted embryonic alkaline phosphatase) reporter genes driven by either the same or two different inducible promoter constructs. Both reporters are readily measurable in the cell culture supernatant when using QUANTI-Luc™, a luciferase detection reagent, and QUANTI-Blue™, a SEAP detection reagent.