RAW-Lucia™ ISG-KO-IRF7 Cells

IRF7 knockout IRF-inducible Lucia luciferase reporter mouse macrophages

RAW-Lucia^™ ISG-KO-IRF7 cells were generated from RAW-Lucia^™ ISG cells through the stable gene knockout of interferon regulatory factor 7 (IRF7), a key transcription regulator of type I interferon (IFN)-dependent immune responses.

These cells derive from the murine RAW 264.7 macrophage cell line, which has been reported to express many pattern recognition receptors (PRRs), including Toll-like receptor 9 (TLR9) [1, 2].

Stimulation of TLR9 with CpG-containing oligonucleotides (CpG-ODNs) induces the formation of a complex consisting of MyD88, TRAF6 and IRF7 leading to the production of type I IFNs.

RAW-Lucia^™ ISG-KO-IRF7 and RAW-Lucia^™ ISG cells can be used to study the role of IRF7 in a mouse macrophage cell line by monitoring of interferon regulatory factor (IRF)-induced Lucia luciferase activity.

They express the gene for secreted Lucia luciferase under the control of the I-ISG54 promoter which is comprised of the IFN-inducible ISG54 promoter enhanced by a multimeric ISRE.

The levels of IRF-induced Lucia in the cell culture supernatant can be easily monitored using QUANTI-Luc^™, a Lucia luciferase detection reagent.

RAW-Lucia^™ ISG-KO-IRF7 cells are resistant to Zeocin^™.

References:

1. Stein SC. et al., 2012. Cell-specific regulation of nucleic acid sensor cascades: a controlling interest in the antiviral response. J Virol. 86(24):13303-12.
2. West AP, Brodsky IE, Rahner C, Woo DK, Erdjument-Bromage H, et al. (2011) TLR signalling augments macrophage bactericidal activity through mitochondrial ROS. Nature 472: 476–480.

Specifications

Antibiotic resistance: Zeocin™

Growth Medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™ supplemented with Zeocin™ 

Quality Control: 
IRF7 knockout is verified by PCR and DNA sequencing to confirm frameshift mutation/deletion. 

The cells are guaranteed mycoplasma-free.

This product is covered by a Limited Use License (See Terms and Conditions).

Contents

• 1 vial containing 3-7 x 10⁶ cells
• 1 ml of Zeocin™ (100 mg/ml)
• 1 ml of Normocin™ (50 mg/ml)
• 1 pouch of QUANTI-Luc™ (Luciferase detection medium)

Shipped on dry ice (Europe, USA & Canada)