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RAW-Lucia™ ISG-KO-IRF7 Cells

RAW-Lucia™ ISG-KO-IRF7 Cells Unit size Cat. code Docs Qty Price
IRF7 Knockout IRF-Lucia Luciferase Reporter Cell Line
3-7 x 10e6 cells
rawl-koirf7
+-
$1,244.00

IRF7 knockout IRF-inducible Lucia luciferase reporter mouse macrophages

RAW-Lucia ISG-KO-IRF7 cells were generated from RAW-Lucia ISG cells through the stable gene knockout of interferon regulatory factor 7 (IRF7), a key transcription regulator of type I interferon (IFN)-dependent immune responses. These cells derive from the murine RAW 264.7 macrophage cell line, which has been reported to express many pattern recognition receptors (PRRs), including Toll-like receptor 9 (TLR9) [1, 2]. Stimulation of TLR9 with CpG-containing oligonucleotides (CpG-ODNs) induces the formation of a complex consisting of MyD88, TRAF6 and IRF7 leading to the production of type I IFNs.

RAW-Lucia ISG-KO-IRF7 and RAW-Lucia ISG cells can be used to study the role of IRF7 in a mouse macrophage cell line by monitoring of interferon regulatory factor (IRF)-induced Lucia luciferase activity. They express the gene for secreted Lucia luciferase under the control of the I-ISG54 promoter which is comprised of the IFN-inducible ISG54 promoter enhanced by a multimeric ISRE. The levels of IRF-induced Lucia in the cell culture supernatant can be easily monitored using QUANTI-Luc, a Lucia luciferase detection reagent.

RAW-Lucia ISG-KO-IRF7 cells are resistant to Zeocin.

 

1. Stein SC. et al., 2012. Cell-specific regulation of nucleic acid sensor cascades: a controlling interest in the antiviral response. J Virol. 86(24):13303-12.
2. West AP, Brodsky IE, Rahner C, Woo DK, Erdjument-Bromage H, et al. (2011) TLR signalling augments macrophage bactericidal activity through mitochondrial ROS. Nature 472: 476–480.

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Specifications

Antibiotic resistance: Zeocin™

Growth Medium: DMEM, 4.5 g/l glucose, 10% fetal bovine serum (FBS), 100 µg/ml Normocin™, 2 mM L-glutamine

Quality Control: 
IRF7 knockout is verified by PCR and DNA sequencing to confirm frameshift mutation/deletion. 

The cells are guaranteed mycoplasma-free.

This product is covered by a Limited Use License (See Terms and Conditions).

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Contents

Shipped on dry ice

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